Recombinant Mouse Antibody (3F4) is capable of binding to Mouse PrP (residue 104-113).
Figure 1 PrPC cleavage analysis.
Immunoblots of truncated proteins from the three PRNP codon 129 genotypes in PBMCs. After PNGase treatment, denatured lysates from 106 PBMCs were tested by Western Blot analysis using 4 antibodies to span the large PrP region: SAF32 (79–91), 8G8 (95–110), 3F4 (109–112) and PRI 917 (216–221). The 27–28 kDa band corresponds to the M.W. of the unglycosylated Full-Length (F.L.) protein, while the bands near 20 kDa and 18 kDa correspond to the C2 and C1 fragments, respectively.
Segarra, C. , Lehmann, S. , & Coste, J. . (2009). Prion protein expression and processing in human mononuclear cells: the impact of the codon 129 prion gene polymorphism. PLOS ONE, 4.
Figure 2 Confocal microscopy of Drosophila S2 cells transiently transfected with mouse 3F4 or hamster PrP constructs.
Drosophila S2 cells were transiently co-transfected with pUASTattB plasmids which contained insert DNA that encoded (a) mouse 3F4 PrP variants; (b) hamster PrP variants or (c) ovine VRQ(GPI), together with the pWA-GAL4-driver plasmid. Cells 24 h post-transfection were fixed with or without prior permeabilisation by treatment with Triton X-100. Mock-transfected cells were treated with the Effectene transfection reagent. Prepared cells were subsequently reacted with the anti-PrP monoclonal antibody 4H11, and additionally with the anti-Golgi polyclonal antibody GM130 in the case of permeabilised cells, prior to confocal microscopy. Nuclei were counterstained with Hoechst. Scale bar: 2 μm.
Thackray, A. M. , Cardova, A. , Wolf, H. , Pradl, L. , & Bujdoso, R. . (2017). Genetic human prion disease modelled in prp transgenic drosophila. Biochemical Journal, 474(19), BCJ20170462.
Figure 3 Histopathologic features of PRNP-Q227X.
Sections are stained with haematoxylin–eosin (a), Luxol-periodic acidSchiff reaction (c), 3F4 anti-PrP antibody (b, d) and anti-tau antibody RD4 (e, f). Original magnification 9200 (a, b, c, d) and 9400 (e, f). Presence of numerous multicentric and unicentric plaques in the frontal cortex (arrows) (a, b). Relative sparing of the cerebellum with sparse amyloid depositions (arrow) (c, d). Dystrophic neurites surrounding PrPSc plaques and neuropil threads (e) and neurofibrillary tangles (f).
Jansen, C. , Parchi, P. , Capellari, S. , Vermeij, A. J. , Corrado, P. , & Baas, F. , et al. (2010). Prion protein amyloidosis with divergent phenotype associated with two novel nonsense mutations inprnp. Acta Neuropathologica, 119(2), 189-197.
Figure 4 Fractions P3 prepared from whole brains of Tg(PG14) or Prnp0/0 mice were perfused for 5 min (bars) over the sensor surface of SPR chips on which antibody 15B3 (A) or 3F4 (B) had been immobilized.
The sensorgrams (time course of the SPR signal in Resonance Units, RU) refer to the specific binding to the antibody (binding was negligible on sensor surfaces without antibody). Significant binding was detected when P3 fractions containing PG14 PrP (black line) were flowed over the 15B3-coated chip (A), but not on the 3F4-coated chip (B). No binding was observed when a P3 fraction from Prnp0/0 mice was analyzed (gray line).
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CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B034(A) | Recombinant Anti-human PRNP Intrabody [(D-Arg)9] | IF, FC, WB, FuncS | scFv-(D-Arg)9 |
IAB-B034(G) | Recombinant Anti-human PRNP Intrabody [+36 GFP] | WB, CO-IP, FuncS | scFv-(+36GFP) |
IAB-B034(T) | Recombinant Anti-human PRNP Intrabody [Tat] | IF, ELISA, FuncS | scFv-Tat |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-310 | Mouse Anti-PRNP Recombinant Antibody (clone POM2) | WB, IHC, FuncS | Mouse IgG |
PABC-107 | Mouse Anti-PRNP Recombinant Antibody (clone ICSM 18) | ELISA, Inhib, IF | Mouse IgG |
PABW-107 | Mouse Anti-PRNP Recombinant Antibody (clone VRQ14) | ELISA | Mouse IgG2a |
FAMAB-0272CQ | Human Anti-PRNP Recombinant Antibody (clone ch31C6) | ELISA, WB, IHC | Chimeric (mouse/human) IgG1 |
FAMAB-0050-CN | Chicken Anti-PRNP Recombinant Antibody (clone phAb4-31) | ELISA | Chicken IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-310 | Mouse Anti-PRNP Recombinant Antibody (clone POM2); scFv Fragment | WB, IHC, FuncS | Mouse scFv |
FAMAB-0294-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone D18); scFv Fragment | WB, IF, IHC | Human scFv |
FAMAB-0295-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone 3:3); scFv Fragment | WB, IF, IHC | Human scFv |
FAMAB-0296-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone 6:4); scFv Fragment | WB, IF, IHC | Human scFv |
PSBC-254 | Mouse Anti-PRNP Recombinant Antibody (clone POM6); scFv Fragment | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-310 | Mouse Anti-PRNP Recombinant Antibody (clone POM2); Fab Fragment | WB, IHC, FuncS | Mouse Fab |
FAMAB-0050-CN-F(E) | Chicken Anti-PRNP Recombinant Antibody (clone phAb4-31); Fab Fragment | ELISA | Chicken Fab |
FAMAB-0051-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone hu4.00); Fab Fragment | ELISA | Humanized Fab |
FAMAB-0052-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone ch4.31); Fab Fragment | ELISA | Chimeric (chicken/human) Fab |
PFBC-254 | Mouse Anti-PRNP Recombinant Antibody (clone POM6); Fab Fragment | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PNBL-026 | Recombinant Anti-Human PRNP VHH Single Domain Antibody (Nb484) | WB, ELISA, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-943LC | Anti-Mouse Prnp Recombinant Antibody (D13) | ELISA | Human antibody |
MRO-944LC | Anti-Mouse Prnp Recombinant Antibody (D18) | ELISA | Human antibody |
MRO-945LC | Anti-Mouse Prnp Recombinant Antibody (R72) | ELISA | Human antibody |
MRO-946LC | Anti-Mouse Prnp Recombinant Antibody (R1) | ELISA | Human antibody |
MRO-947LC | Anti-Mouse Prnp Recombinant Antibody (R2) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-942LC-S(P) | Anti-Sheep PRNP Recombinant Antibody scFv Fragment (6.1) | ELISA | Mouse antibody |
MRO-948LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (6H10) | ELISA | Mouse antibody |
MRO-949LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (31C6) | ELISA | Mouse antibody |
MRO-950LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (72-5) | ELISA | Mouse antibody |
MRO-951LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (44B1) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0727MZ | Chicken Anti-PRNP Polyclonal IgY | ICC/IF | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2871 | Hi-Affi™ Recombinant Rabbit Anti-PRNP Monoclonal Antibody (DS2871AB) | WB, FC, IHC-P, ICC/IF | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0659LC | Recombinant Mouse Anti-Human PRNP Antibody (4F2) | WB | IgG |
EPAF-0276CQ | Mouse Anti-PRNP Recombinant Antibody (clone E149) | FC, ELISA | Mouse IgG |
EPAF-0277CQ | Mouse Anti-PRNP Recombinant Antibody (clone 4H8) | Inhib | Mouse IgG2b |
EPAF-0278CQ | Mouse Anti-PRNP Recombinant Antibody (clone 6BH) | Inhib | Mouse IgG1 |
EPAF-0279CQ | Mouse Anti-PRNP Recombinant Antibody (clone 3F10) | WB | Mouse IgG1 |
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