Recombinant Mouse Anti-P. falciparum AMA1 Antibody (1F9) (CAT#: PABW-097)

Recombinant Mouse Antibody (1F9) is capable of binding to P. falciparum AMA1, expressed in Chinese Hamster Ovary cells (CHO). This antibody could inhibit merozoite invasion by several mechanisms.


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Figure 1 ELISA analysis of MAb 1F9, 5G8, 2C5 reactivity with recombinant refolded AMA1 derived from P. falciparum strains 3D7, D10, HB3, and W2mef. AMA1 preparations were adsorbed onto the wells, and antibodies were then allowed to react at the indicated concentrations.

Figure 1 ELISA analysis of MAb 1F9, 5G8, 2C5 reactivity with recombinant refolded AMA1 derived from P. falciparum strains 3D7, D10, HB3, and W2mef. AMA1 preparations were adsorbed onto the wells, and antibodies were then allowed to react at the indicated concentrations.

Absorbances at 450 nm and MAb concentrations are indicated on the ordinate and abscissa, respectively.

Coley, A. M. . (2006). The most polymorphic residue on plasmodium falciparum apical membrane antigen 1 determines binding of an invasion-inhibitory antibody. Infection & Immunity, 74(5), 2628.

Figure 2 Western analysis of AMA1 expressed by parasite strains 3D7, D10, HB3, and W2mef. Schizont-stage parasites were separated by SDS-PAGE and blotted onto polyvinylidene difluoride, and blots were visualized with Mabs 1F9, 2C5, and 5G8 and rabbit anti-AMA1.

Figure 2 Western analysis of AMA1 expressed by parasite strains 3D7, D10, HB3, and W2mef. Schizont-stage parasites were separated by SDS-PAGE and blotted onto polyvinylidene difluoride, and blots were visualized with Mabs 1F9, 2C5, and 5G8 and rabbit anti-AMA1.

Arrows indicate the locations of the unprocessed 83-kDa and processed 66-kDa bands.

Coley, A. M. . (2006). The most polymorphic residue on plasmodium falciparum apical membrane antigen 1 determines binding of an invasion-inhibitory antibody. Infection & Immunity, 74(5), 2628.

Figure 3 Growth inhibition assays of 3D7, D10, HB3, and W2mef parasites.

Figure 3 Growth inhibition assays of 3D7, D10, HB3, and W2mef parasites.

Each antibody, i.e., 1F9 (A), 2C5 (B), and rabbit anti-AMA1 (C), was assayed for its growth inhibition qualities. Data points indicate the means of three identical wells, and error bars indicate one standard deviation from the mean.

Coley, A. M. . (2006). The most polymorphic residue on plasmodium falciparum apical membrane antigen 1 determines binding of an invasion-inhibitory antibody. Infection & Immunity, 74(5), 2628.


Specifications

  • Immunogen
  • P. falciparum apical membrane antigen 1
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native P. falciparum AMA1
  • Species Reactivity
  • P. falciparum
  • Clone
  • 1F9
  • Applications
  • ELISA, BL

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The AMA1 antibody has been reported in applications of ELISA, WB, Inhibition.
    WB: The membrane was blocked with 10% skim milk in phosphatebuffered saline (PBS) and developed with 2 ug ml 1 of either MAb 1F9, 2C5, or 5G8 or a pool of rabbit anti-3D7, -D10, -HB3, and -W2mef AMA1 antisera in PBS, followed by 1g ml 1 peroxidase-conjugated rabbit anti-mouse or goat anti-rabbit immunoglobulin G (Chemicon) accordingly.
    ELISA: For assays involving binding of recombinant refolded AMA1 to MAbs 1F9, 2C5, and 5G8, 100 l AMA1 (1 ug/ml) in coating buffer (0.1 M sodium carbonate, pH 9.5) was applied to Maxisorp (Nunc) enzyme-linked immunosorbent assay (ELISA) plate wells overnight at 4°C.

Target

  • Alternative Names
  • AMA1; apical membrane antigen 1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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