Human Anti-JAG1 Recombinant Antibody (clone b70) (CAT#: PABL-312)
Recombinant Human Antibody (b70) is capable of binding to Protein jagged-1, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Protein jagged-1 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Protein jagged-1 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
IB
Figure 1 Immunoblot analysis of NOTCH2 intracellular domain (NICD2) in U87 glioblastoma cells to assess selectivity of JAG1 inhibition.
Anti-JAG1.b70 (25 μg/ ml) completely blocked NOTCH2 signalling.
Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127-131.
IF
Figure 2 Immunofluorescence staining of bronchiolar epithelium for CC10 (green) and FOXJ1 (red) from mice dosed every 3 days over 8 days with the anti-JAG1.b70 antibody.
Nuclei were counterstained with DAPI.
Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127-131.
IHC
Figure 3 Haematoxylin and eosin staining of skin from mice treated with anti-JAG1.b70.
JAG2 but not JAG1 inhibition induced a loss of mature sebocytes in sebaceous glands.
Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127-131.
IF
Figure 4 Representative immunofluorescence images (BrdU, green) of sections used for the quantification.
Percentages of BrdU-positive airway cells (n = 5 mice per group).
Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127-131.
Specifications
- Immunogen
- Human jagged 1
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- Human JAG1
- Species Reactivity
- Human
- Clone
- b70
- Applications
- IB, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunoblotting, Immunofluorescence and Immunohistochemistry.
Target
- Alternative Names
- JAG1; jagged 1; AGS; AHD; AWS; HJ1; CD339; JAGL1; protein jagged-1
- Gene ID
- 182
- UniProt ID
- P78504
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-617 | Human Anti-JAG1 Recombinant Antibody; Fab Fragment | WB, ELISA, FuncS | Human Fab |
| NS-049CN-F(E) | Human Anti-JAG1 Recombinant Antibody; Fab Fragment (NS-049CN-F(E)) | ELISA, WB, IF | Human Fab |
| HPAB-0947LY-F(E) | Human Anti-JAG1 Recombinant Antibody; Fab Fragment (HPAB-0947LY-F(E)) | ELISA | Human Fab |
| HPAB-0948LY-F(E) | Human Anti-JAG1 Recombinant Antibody; Fab Fragment (HPAB-0948LY-F(E)) | ELISA | Human Fab |
| HPAB-0949LY-F(E) | Human Anti-JAG1 Recombinant Antibody; Fab Fragment (HPAB-0949LY-F(E)) | ELISA | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0602CL | Mouse Anti-JAG1 Recombinant Antibody (TAB-0602CL) | FC, DB | Mouse IgG |
| TAB-0603CL | Mouse Anti-JAG1 Recombinant Antibody (TAB-0603CL) | Block, DB, ELISA | Mouse IgG |
| TAB-0604CL | Human Anti-JAG1 Recombinant Antibody (TAB-0604CL) | ELISA | Human antibody |
| TAB-0605CL | Human Anti-JAG1 Recombinant Antibody (TAB-0605CL) | ELISA | Human antibody |
| PABX-126-S (P) | Recombinant Human Anti-Jag1 Antibody scFv Fragment (JAG2.b33) | WB, Neut, FuncS | scFv |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-126-F (E) | Recombinant Human Anti-Jag1 Antibody Fab Fragment (JAG2.b33) | WB, Neut, FuncS | Fab |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1607CQ | Recombinant Mouse Anti-JAG1 Antibody (CBL125) | FC, WB, Neut | IgG2 |
| NEUT-1608CQ | Recombinant Mouse Anti-JAG1 Antibody (4A24) | FC, Neut | IgG2 |
| NEUT-1609CQ | Recombinant Mouse Anti-JAG1 Antibody (CBL581) | Neut | IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1895 | Hi-Affi™ Recombinant Rabbit Anti-JAG1 Monoclonal Antibody (DS1895AB) | WB, IHC-P, ICC/IF | IgG |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-049CN | Human Anti-JAG1 Recombinant Antibody (NS-049CN) | ELISA, WB, IF | Human IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1018LY-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-1018LY-S(P)) | ELISA | Human scfv |
| HPAB-1019LY-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-1019LY-S(P)) | ELISA | Human scfv |
| HPAB-1020LY-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-1020LY-S(P)) | ELISA | Human scfv |
| HPAB-1021LY-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-1021LY-S(P)) | ELISA | Human scfv |
| HPAB-0474-FY-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-0474-FY-S(P)) | ELISA | Human scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0957LY | Human Anti-JAG1 Recombinant Antibody (HPAB-0957LY) | ELISA | Human IgG |
| HPAB-0958LY | Human Anti-JAG1 Recombinant Antibody (HPAB-0958LY) | ELISA | Human IgG |
| HPAB-0959LY | Human Anti-JAG1 Recombinant Antibody (HPAB-0959LY) | ELISA | Human IgG |
| HPAB-0960LY | Human Anti-JAG1 Recombinant Antibody (HPAB-0960LY) | ELISA | Human IgG |
| HPAB-0961LY | Human Anti-JAG1 Recombinant Antibody (HPAB-0961LY) | ELISA | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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