Human Anti-RSPO3 Recombinant Antibody (clone h131R007) (CAT#: HPAB-0037CQ)

Figure 1 Inhibition of B-catenin signaling induced by RSPO3 and WNT3A.

A TOPflash luciferase reporter assay was used to measure B-catenin signaling in HEK-293T cells after exposure to a combination of WNT3a and human RSPO3 and in the presence of increasing concentrations of anti-RSPO3 antibody 131R007 (-□-) or 131R010 (-●-). Antibodies were used as 5-fold serial dilutions from 20 μg/mL to 0.0064 g/ml. Controls included exposure to control medium (no WNT3a and no RSPO/cells (-▲-)), WNT3a alone (-▼-), or a combination of WNT3a and human RSPO3 in the absence of antibody (-♦-).

Figure 2 Inhibition of RSPO3 and LGR5 interaction by anti-RSPO3 antibodies.

FACS analysis of HEK-293T cells expressing LGR5. HEK-293T cells were transiently trans fected with a cDNA expression vector encoding the extracel lular domain of human LGR5 (FLAG-LGR5-CD4TM-GFP) and then subsequently mixed with RSPO3-biotin fusion pro tein in combination with anti-RSPO3 antibodies 131 R006 or 131R007. Binding was detected with PE-conjugated strepta vidin. Relative RSPO3-biotin binding is shown on the y-axis and expression of the FLAG-LGR5-CD4TM-GFP fusion protein is indicated on the X-axis. Positive binding is indicated by the presence of signal within the dark lined box overlay on each FACS plot.