Mouse Anti-CTNNB1 Recombinant Antibody (clone BC-22) (CAT#: PABZ-140)

Recombinant Mouse Antibody (BC-22) is capable of binding to Rabbit CTNNB1 (residue 19-44), expressed in Chinese Hamster Ovary cells (CHO). The peptide's epitope is predominantly located in the large bend and in the N-terminal segment, implicating bidentate association.


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Figure 1 HGF enhances anti-Fas-induced apoptosis likely at the level significantly reduced in the presence of HGF, of caspase-8 activation.

Figure 1 HGF enhances anti-Fas-induced apoptosis likely at the level significantly reduced in the presence of HGF, of caspase-8 activation.

A: Jurkat cells deficient in caspase-8 (JB6) and its caspase-8 reconstituted subline (BC-22) were treated with CH11 anti-Fas antibody (100 ng/ml) with or without HGF (0.5 mg/ml) for 12 h. Cell death was assessed by trypan blue staining (mean ± s.d.). B: Different Jurkat cell lines as indicated were treated with medium (black column), etoposide (20 mM, gray column) or etoposide plus 0.5 mg/ml of HGF (white column) for 12 h and analyzed for cell death by the trypan blue assay (mean W s.d.).

Zhao, Y., DiFrancesca, D., Wang, X. U. E., Zarnegar, R., Michalopoulos, G. K., & Yin, X. M. (2007). Promotion of Fas‐mediated apoptosis in Type II cells by high doses of hepatocyte growth factor bypasses the mitochondrial requirement. Journal of cellular physiology, 213(2), 556-563.

Figure 2 Binding of mAb BC-22 to the phosphorylated P-b-Cat19–44 peptide

Figure 2 Binding of mAb BC-22 to the phosphorylated P-b-Cat19–44 peptide

Megy, S., Bertho, G., Gharbi-Benarous, J., Baleux, F., Benarous, R., & Girault, J. P. (2006). STD and TRNOESY NMR studies for the epitope mapping of the phosphorylation motif of the oncogenic protein β-catenin recognized by a selective monoclonal antibody. FEBS letters, 580(22), 5411-5422.

Figure 3 Epitope mapping of the P-β-Cat<sup>19–44</sup> peptide in the presence of mAb, for a peptide:antibody ratio of 100:1.

Figure 3 Epitope mapping of the P-β-Cat19–44 peptide in the presence of mAb, for a peptide:antibody ratio of 100:1.

Expansion of the region containing resonances of the amide protons: Reference 1D ¹ H spectrum (in black) of the P-β-Cat19–44 peptide in association with the mAb antibody and 1D ¹ H STD NMR spectrum (in red) of the P-β-Cat19–44 peptide in association with the mAb antibody, showing enhancements of resonances of protons making close contacts with the antibody-combining site. STD values are obtained after peak picking intensities compared to the 1D with the exact values of chemical shifts of the HN (in bold: amino acids with intense relative STD, and in italic: amino acids with weak relative STD). Variations of the chemical shifts between the HN are equal to or higher than 0.01 ppm. There are only Ala20 and Ser23, which pose problem since they leave together to 8.52 ppm. The value given can thus correspond only to the average of relative STD value found for each one.

Megy, S., Bertho, G., Gharbi-Benarous, J., Baleux, F., Benarous, R., & Girault, J. P. (2006). STD and TRNOESY NMR studies for the epitope mapping of the phosphorylation motif of the oncogenic protein β-catenin recognized by a selective monoclonal antibody. FEBS letters, 580(22), 5411-5422.


Specifications

  • Immunogen
  • Rabbit catenin (cadherin-associated protein), beta 1, 88kDa
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Rabbit CTNNB1
  • Species Reactivity
  • Rabbit
  • Clone
  • BC-22
  • Applications
  • ELISA, Block

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody BC-22 has been reported in applications of Enzyme-linked Immunosorbent Assay, Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

  • Alternative Names
  • CTNNB1; catenin (cadherin-associated protein), beta 1, 88kDa; beta-catenin;

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone BC-22"

See other products for "CTNNB1"

Single-domain Antibody

CAT Product Name Application Type
NABG-049 Recombinant Anti-Mouse Ctnnb1 VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH

Rabbit Monoclonal Antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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