Mouse Anti-MOG Recombinant Antibody (clone 8-18C5) (CAT#: PABZ-152)

Figure 1 Fluorescence intensity of the MOG-transfected cell line (TE 671) is shown by staining the cells with the monoclonal antibody (mAb) anti-MOG 8–18C5 antibody.

Pröbstel, A. K., Dornmair, K., Bittner, R., Sperl, P., Jenne, D., Magalhaes, S., ... & Krumbholz, M. (2011). Antibodies to MOG are transient in childhood acute disseminated encephalomyelitis. Neurology, WNL-0b013e318228c0b1.

Figure 2 Analysis of antibody binding to h-MOG expressing fibroblasts by confocal microscopy and FACS.

Untransfected LTK⁻ cells are negative when stained with mAb 8-18C5. b MOG-LTK⁻ transfectants exhibit intense red surface staining after incubation with 8-18C5. Nuclei are counterstained green with Syto13. The scale bars indicate 20 μm. c and d show the corresponding FACS staining with the anti-MOG mAb 8-18C5, demonstrating a clear signal for the transfectants. All the mouse mAbs exhibited the same staining pattern.

Brehm, U., Piddlesden, S. J., Gardinier, M. V., & Linington, C. (1999). Epitope specificity of demyelinating monoclonal autoantibodies directed against the human myelin oligodendrocyte glycoprotein (MOG). Journal of neuroimmunology, 97(1-2), 9-15.

Figure 3 Western blot analysis of antibody binding to recombinant human MOG-Ig

mAb 8-18C5 only recognised the intact Ig-domain of MOG, as did mAbs Y1, Y2, Y8, Y9, Z2, Z4 and Z12.

Brehm, U., Piddlesden, S. J., Gardinier, M. V., & Linington, C. (1999). Epitope specificity of demyelinating monoclonal autoantibodies directed against the human myelin oligodendrocyte glycoprotein (MOG). Journal of neuroimmunology, 97(1-2), 9-15.

Figure 4 Recognition of h-MOG peptides by mAbs in ELISA

Brehm, U., Piddlesden, S. J., Gardinier, M. V., & Linington, C. (1999). Epitope specificity of demyelinating monoclonal autoantibodies directed against the human myelin oligodendrocyte glycoprotein (MOG). Journal of neuroimmunology, 97(1-2), 9-15.