Recombinant Mouse Antibody (RAC18) is capable of binding to Ricinus communis ricin. RAC18's binding of this epitope is consistent with its ability to inhibit the enzymatic function of RTA in vitro.
Figure 1 Protective effects of Ab-DeliverIN (DelIN)-mediated intracellular delivery of polyclonal anti-deglycosylated ricin A-chain antibody (anti-dgA Ab)
(A) and monoclonal RAC18 anti-ricin A-chain monoclonal antibody (RAC18 mAb) (B) against ricin-induced cytotoxicity. RAW264.7 cells were incubated with naked antibodies (dgA Ab, RAC18 mAb or control IgG) (no liposome encapsulation) or DelIN-encapsulated antibodies (DelIN-dgA Ab, DelI-RAC18 mAb or DelIN-control IgG) for 8 h. After 3 washes with PBS to remove extracellular antibodies, ricin (100 ng/mL) was added and incubated for additional 24 h. Cell viability was quantitated using the MTT assay. As controls for the neutralizing effects of anti-ricin antibodies, ricin was co-incubated with naked dgA Ab, RAC18 mAb or control IgG.
Wu, F., Fan, S., Martiniuk, F., Pincus, S., Müller, S., Kohler, H., & Tchou-Wong, K. M. (2010). Protective effects of anti-ricin A-chain antibodies delivered intracellularly against ricin-induced cytotoxicity. World journal of biological chemistry, 1(5), 188-195.
Figure 2 Intracellular localization of fluorescent-labeled ricin and fluorescent-labeled DelIN-encapsulated RAC18 mAb by immunofluorescent staining.
Immunofluorescent staining of RAW264.7 cells treated with Alexa 488-labeled ricin and DelIN-encapsulated Alexa 594-labeled RAC18 mAb. A: Alexa 488-labeled ricin (green); B: DelIN-Alexa 594-labeled RAC18 mAb (red); C: Superposition of A and B demonstrated co-localization of ricin and RAC18 mAb (yellow); D: Immunofluorescent staining of RAW264.7 cells treated with naked Alexa 594-labeled RAC18 mAb. No staining was observed, demonstrating the inability of naked RAC18 mAb to enter cells.
Wu, F., Fan, S., Martiniuk, F., Pincus, S., Müller, S., Kohler, H., & Tchou-Wong, K. M. (2010). Protective effects of anti-ricin A-chain antibodies delivered intracellularly against ricin-induced cytotoxicity. World journal of biological chemistry, 1(5), 188-195.
Figure 3 Ricin accumulates at the cell surface in the presence of neutralizing Ab.
Top. Live cells were incubated with labeled transferrin (red) and ricin (green) for 10 min in the presence of the indicated antibodies, or no antibody, and then fixed. Cells were imaged via confocal microscopy, and a central slice from a z stack is shown. In the absence of Ab (left), ricin and transferrin enter the cells by similar processes and colocalize. But in the presence of the nAb RAC18 (center), ricin is retained at the cell surface, while transferrin still enters. Non-neutralizing Ab RAC23 (right) has no effect on ricin internalization. Full z stacks of each of these photos are shown in videos S3, S4, S5, S6. These experiments have been replicated in three individual experiments, with>100 cells visualized. Bottom. The increase of cell surface ricin is confirmed, and quantified, by flow cytometry. Cells were incubated with the indicated Ab and ricin for 60 min either in the cold with sodium azide, or at 37° in tissue culture medium. The former studies passive binding at the cell surface, the latter also allows for internalization. 10,000 cells were analyzed. In either case RAC 18 results in approximately two-fold increase in cell-associated ricin.
Song, K., Mize, R. R., Marrero, L., Corti, M., Kirk, J. M., & Pincus, S. H. (2013). Antibody to ricin a chain hinders intracellular routing of toxin and protects cells even after toxin has been internalized. PLoS One, 8(4), e62417.
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CAT | Product Name | Application | Type |
---|---|---|---|
PFBZ-192 | Mouse Anti-Ricin Recombinant Antibody (clone RAC18); Fab Fragment | Neut | Mouse Fab |
PSBL-685 | Mouse Anti-Ricin Recombinant Antibody (clone mAb RAC18); scFv Fragment | Neut | Mouse scFv |
FAMAB-0060JF-S(P) | Monkey Anti-Ricin Recombinant Antibody (clone 43RCA); scFv Fragment | WB, ELISA, FuncS | Monkey scFv |
PSBS-0050 | Mouse Anti-Ricin Recombinant Antibody; scFv Fragment (PSBS-0050) | ELISA, Neut, FuncS | Mouse scFv |
PSBS-0068 | Human Anti-Ricin Recombinant Antibody; scFv Fragment (PSBS-0068) | ELISA, Neut, FuncS | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-685 | Mouse Anti-Ricin Recombinant Antibody (clone mAb RAC18); Fab Fragment | Neut | Mouse Fab |
PSBZ-192 | Mouse Anti-Ricin Recombinant Antibody (clone RAC18); scFv Fragment | Neut | Mouse scFv |
FAMAB-0060JF-F(E) | Monkey Anti-Ricin Recombinant Antibody (clone 43RCA); Fab Fragment | WB, ELISA, FuncS | Monkey Fab |
FAMAB-0310JF-F(E) | Mouse Anti-Ricin Recombinant Antibody (clone 3E1); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
PFBS-0050 | Mouse Anti-Ricin Recombinant Antibody; Fab Fragment (PFBS-0050) | ELISA, Neut, FuncS | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-165 | Recombinant Mouse Anti-Ricin Antibody (RAC18) | FuncS, IF | IgG |
PABX-165-S (P) | Recombinant Mouse Anti-Ricin Antibody scFv Fragment (RAC18) | Neut, FuncS | scFv |
PABX-165-F (E) | Recombinant Mouse Anti-Ricin Antibody Fab Fragment (RAC18) | Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1607-FY | Human Anti-Ricin Recombinant Antibody (clone REV8) | WB, ELISA, IP | Chimeric (macaque/human) IgG |
HPAB-1608-FY | Human Anti-Ricin Recombinant Antibody (clone REV7) | WB, ELISA, IP | Chimeric (macaque/human) IgG |
FAMAB-0060JF | Monkey Anti-Ricin Recombinant Antibody (clone 43RCA) | WB, ELISA, IHC, Neut | Monkey IgG |
FAMAB-0310JF | Mouse Anti-Ricin Recombinant Antibody (clone 3E1) | WB, ELISA, IHC | Mouse IgG |
FAMAB-0311WJ | Camelid Anti-Ricin Recombinant Antibody (clone LRE7) | ELISA, Neut | Camelid VHH |
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