Anti-S. agalactiae FbsA protein Recombinant Antibody (10H1) (MRO-131MZ) (CAT#: MRO-131MZ)

This monoclonal antibody 10H1 which binds to the FbsA protein of Streptococcus agalactia may be useful in the treatment and protection against infections from S. agalactiae.


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Inhib

Figure 1 Inhibition of Fng binding to 6pGST by the 5H2mAb.

Figure 1 Inhibition of Fng binding to 6pGST by the 5H2mAb.

Goat anti-GST was immobilized on the wells of microtiterplates, followed by incubation with 6pGST (100 nM). Fng (500nM) was mixed with 1 µg/ml of mAb 5H2, mAb 10H1 or mouseIgG1 (isotype control) before being added to the wells. Afterwashing, Fng binding was detected using rabbit anti-Fngantibodies followed by alkaline phosphatase-conjugated goatanti-rabbit IgG.

Immunogenic Properties of Streptococcus agalactiae FbsA Fragments

Inhib

Figure 2 Inhibition of GBS attachment to surface-coatedFng by the 5H2 mAb.

Figure 2 Inhibition of GBS attachment to surface-coatedFng by the 5H2 mAb.

Cells of S. agalactiae 6313 (5x107) werepreincubated with the indicated amounts of mAbs 5H2 or 10H1,transferred to Fng-coated wells (1 µg/well) and the mixtureswere incubated for 2 hours. After extensive washes, 1 µg rabbitanti-GBS IgG was added to the wells, followed by a 90 minincubation. Adherent bacteria were detected by peroxidase-conjugated goat anti-rabbit IgG and the plates were developedwith o-phenylenediamine. All the data are expressed aspercentages of control adherence, where bacteria attachmentin the absence of antibody was set to 100% (equivalent to 0%inhibition). The bars show standard deviations of triplicatesamples. This experiment was performed three times withsimilar results.

Immunogenic Properties of Streptococcus agalactiae FbsA Fragments


Specifications

  • Host Species
  • Mouse
  • Specificity
  • Streptococcus agalactiae
  • Clone
  • 10H1
  • Applications
  • WB, ELISA, Inhib
  • Related Disease
  • Streptococcus agalactiae infections

Applications

  • Application Notes
  • The S. agalactiae FbsA Protein antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Inhibition.

Target

  • Alternative Names
  • S. agalactiae FbsA protein

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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