Recombinant Mouse Anti-S. flexnerisero type 2a Antibody scFv Fragment (F22-4) (CAT#: PABX-168-S (P))

Anti-S. flexnerisero type 2a antibody is a Mouse of scFv class that binds to an S. flexnerisero type 2a. The antibody has shown in a mouse model that they protect against homologous, but not heterologous, challenge.


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Figure 1 Conformational energy maps of glycosidic linkages calculated from MD simulations of (a) D0AB(E)CD and (b) ECDA′B′ in complex with mAb F22-4 and (c) D0AB(E)C and (d) ECDA′B′ free in solution.

Figure 1 Conformational energy maps of glycosidic linkages calculated from MD simulations of (a) D0AB(E)CD and (b) ECDA′B′ in complex with mAb F22-4 and (c) D0AB(E)C and (d) ECDA′B′ free in solution.

Dihedral angles are defined as Φ = O5-C1-O1-Cx and Ψ = C1-O1-Cx-Cx−1. Contours are separated by 0.75 kcal. mol−1. The represented conformational behaviors are representative of those observed with other branched and linear oligosaccharides.

Theillet, F. X., Frank, M., Vulliez-Le Normand, B., Simenel, C., Hoos, S., Chaffotte, A., ... & Mulard, L. A. (2011). Dynamic aspects of antibody: oligosaccharide complexes characterized by molecular dynamics simulations and saturation transfer difference nuclear magnetic resonance. Glycobiology, 21(12), 1570-1579.

Figure 2 Views showing the interaction between F22-4 and [AB(E)CD]2 (see Fig. S2 for stereoview).

Figure 2 Views showing the interaction between F22-4 and [AB(E)CD]2 (see Fig. S2 for stereoview).

(A) A detailed view of [AB(E)CD]2 bound to F22-4. Rha residues are in purple, Glc are in blue and GlcNAc are in green. The antibody is shown in ribbon form with residues hydrogen bonding to the ligand in atomic form. Antibody–antigen hydrogen bonds are shown as dashed lines.
(B) View from above the antigen-binding site. The variable domains of F22-4 are shown in surface representation with the VH domain in purple and the VL domain in blue. [AB(E)CD]2 is shown in atomic form with carbon in yellow, oxygen in red, and nitrogen in blue. Water molecules bound to [AB(E)CD]2 (and in some cases to the antibody as well) are shown as red spheres.

Vulliez-Le Normand, B., Saul, F. A., Phalipon, A., Belot, F., Guerreiro, C., Mulard, L. A., & Bentley, G. A. (2008). Structures of synthetic O-antigen fragments from serotype 2a Shigella flexneri in complex with a protective monoclonal antibody. Proceedings of the National Academy of Sciences, 105(29), 9976-9981.


Specifications

  • Immunogen
  • S. flexneri serotype 2a
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • scFv
  • Specificity
  • Tested positive against native HIV-1
  • Species Reactivity
  • Shigella flexneri serotype 2a
  • Clone
  • F22-4
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:10000-1:20000
    FuncS (assay dependent)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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