Anti-Human S100A4 Recombinant Antibody (TAB-378CT) (CAT#: TAB-378CT)

Recombinant monoclonal antibody specifically binds to human S100A4 (ELPSFLGKRT). This antibody has anti- angiogenic activity.


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  • Conjugation:
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  • Published Data
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
FuncS

Figure 1 Antitumor activity of 5C3.

Figure 1 Antitumor activity of 5C3.

PBS (negative control) or 5C3 (25 mg/kg) was given i.p. three times a week (1010100). At the end of the experiment, mice were sacrificed and tumors were weighted. Graphs of RTV shows the activity of 5C3 compared with the control group. Bars of tumor weight show the mean ± SEM. *p<0.05.

Hernandez, J. L., Padilla, L., Dakhel, S., Coll, T., Hervas, R., Adan, J., ... & Rodríguez, L. (2013). Therapeutic targeting of tumor growth and angiogenesis with a novel anti-S100A4 monoclonal antibody. PloS one, 8(9), e72480.

IHC

Figure 2 Immunohistologic analysis of tumor microvasculature from MiaPACA-2 tumors in animals treated with 5C3.

Figure 2 Immunohistologic analysis of tumor microvasculature from MiaPACA-2 tumors in animals treated with 5C3.

Quantifications were made from more than 10 pictures per slice at a magnification of X120 (2.4 mm²). Graphs show the mean ± SEM. *p<0.05.

Hernandez, J. L., Padilla, L., Dakhel, S., Coll, T., Hervas, R., Adan, J., ... & Rodríguez, L. (2013). Therapeutic targeting of tumor growth and angiogenesis with a novel anti-S100A4 monoclonal antibody. PloS one, 8(9), e72480.

Neut

Figure 3 5C3 (1-2 mM) neutralized the production of active forms of MMP-9 induced by S100A4 (arrowhead).

Figure 3 5C3 (1-2 mM) neutralized the production of active forms of MMP-9 induced by S100A4 (arrowhead).

Each data point was normalized to the positive control of cells incubated with complete medium (left bar) that represents 100% migration. The control is the maximum of the possible migration (migration control). This migration is obtained by incubating HUVEC with basal medium containing 10% FCS plus hydrocortisone, brain bovine extract and hEGF. All the experimental points are referred to this control.

Hernandez, J. L., Padilla, L., Dakhel, S., Coll, T., Hervas, R., Adan, J., ... & Rodríguez, L. (2013). Therapeutic targeting of tumor growth and angiogenesis with a novel anti-S100A4 monoclonal antibody. PloS one, 8(9), e72480.

Inhib

Figure 4 Dose-response inhibition of S100A4-RAGE interaction by 5C3 mAb.

Figure 4 Dose-response inhibition of S100A4-RAGE interaction by 5C3 mAb.

Antibody was used at 60–500 nM for 2 mM S100A4.

Hernandez, J. L., Padilla, L., Dakhel, S., Coll, T., Hervas, R., Adan, J., ... & Rodríguez, L. (2013). Therapeutic targeting of tumor growth and angiogenesis with a novel anti-S100A4 monoclonal antibody. PloS one, 8(9), e72480.


Specifications

  • Immunogen
  • Full-length human S100A4
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Human S100A4
  • Species Reactivity
  • Human, Mouse
  • Applications
  • FuncS, IHC, Neut, Inhib
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein A/G purified
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Functional Assay, Immunohistochemistry, Neutralization, Inhibition.

Target

  • Alternative Names
  • S100A4; S100 calcium binding protein A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98; protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein; calvasculin; metastasin; murine placental homolog); LK-1

Recommended Products

Reconstitution Buffer

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "S100A4"

Humanized Antibody

CAT Product Name Application Type
TAB-546CL Human Anti-S100A4 Recombinant Antibody (TAB-546CL) ELISA Human IgG

Single-domain Antibody

CAT Product Name Application Type
TAB-377CT Anti-Human S100A4 Therapeutic Single Domain Antibody (aMTS1) WB, ELISA Single domain antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3155 Hi-Affi™ Recombinant Rabbit Anti-S100A4 Monoclonal Antibody (DS3155AB) WB, IHC, ICC, IP IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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