Anti-Human SIRPA Recombinant Antibody (AM4-1) (CAT#: TAB-454CT)

Recombinant monoclonal antibody AM4-1 specifically binds to SIRPα and can block engagement of SIRPa by CD47The antibody can be potentially used in treating hematological cancer.


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SDS-PAGE

Figure 1 Anti-SIRPA Recombinant Antibody (TAB-454CT) in SDS-PAGE

Figure 1 Anti-SIRPA Recombinant Antibody (TAB-454CT) in SDS-PAGE

SDS-PAGE analysis of TAB-454CT in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.

SEC-HPLC

Figure 2 Anti-SIRPA Recombinant Antibody (TAB-454CT) in SEC-HPLC

Figure 2 Anti-SIRPA Recombinant Antibody (TAB-454CT) in SEC-HPLC

SEC-HPLC analysis of TAB-454CT shows that the purity is more than 95%.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

WB

Figure 3 Anti-SIRPA Recombinant Antibody (TAB-454CT) in WB

Figure 3 Anti-SIRPA Recombinant Antibody (TAB-454CT) in WB

Western blot analysis of TAB-454CT was performed by loading human SIRPA protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-454CT and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.6 μg)

DB

Figure 4 Anti-SIRPA Recombinant Antibody (TAB-454CT) in DB

Figure 4 Anti-SIRPA Recombinant Antibody (TAB-454CT) in DB

Dot Blot analysis of TAB-454CT was performed by coating with human SIRPA protein (His tag).

TAB-454CT incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)

ELISA

Figure 5 Anti-SIRPA Recombinant Antibody (TAB-454CT) in ELISA

Figure 5 Anti-SIRPA Recombinant Antibody (TAB-454CT) in ELISA

ELISA analysis of TAB-454CT was performed by coating with human SIRPA protein (His tag). Then blocked with BSA and incubated with TAB-454CT. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Host Species
  • Human
  • Derivation
  • Library F
  • Type
  • Human antibody
  • Specificity
  • Human
  • Species Reactivity
  • Human
  • Clone
  • AM4-1
  • Applications
  • Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay
  • Related Disease
  • Hematologic cancers

Target

  • Alternative Names
  • SIRPA; signal-regulatory protein alpha; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; anti-SIRPA

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone AM4-1"

See other products for "Sirpa"

Single-domain Antibody

CAT Product Name Application Type
NAB-1201-VHH Recombinant Anti-Mouse Sirpa VHH Single Domain Antibody IP, FC, ChiP, Neut Llama VHH

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0465MZ Chicken Anti-PTPNS1 Polyclonal IgY WB Chicken antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-1970CQ Recombinant Rat Anti-Sirpa Antibody (P84) FC, BL, IF, IHC, IP IgG1, κ

Neutralizing Antibody

CAT Product Name Application Type
NEUT-1971CQ Recombinant Mouse Anti-Sirpa Antibody (CBL650) WB, FC, CyTOF, Neut IgG2b

Rabbit Monoclonal Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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