Recombinant Mouse Antibody (BV04-01) is capable of binding to Single-stranded DNA.
Figure 1 Inhibition of horseradish peroxidase-labeled mAb fragments, and SCA 04-01.
Polystyrene plates were coated with ssDNA and co-incubated with horseradish peroxidase-BV04-01 and the following inhibitors, mAb BV04-01 (triangles), BV04-01 Fab fragments (circles), and SCA 04-01 (squares).
Rumbley, C. A., Denzin, L. K., Yantz, L. S. Y. T., Tetin, S. Y., & Voss, E. W. (1993). Construction, characterization, and selected site-specific mutagenesis of an anti-single-stranded DNA single-chain autoantibody. Journal of Biological Chemistry, 268(18), 13667-13674.
Figure 2 Anti-ssDNA activity of BiSCA protein determined in the ELISA.
Individual points represent mean values of triplicate trials with standard deviations (error bars). Points lacking error bars indicate standard deviations smaller than symbol. A, anti-ssDNA activity of fluorescein-purified BiSCA. Polystyrene wells were coated with ssDNA and incubated with BiSCA (10 μg/ml starting concentration) liganded with fluorescein. BiSCA binding to ssDNA was detected with 1A4 (non-ligand-inhibitable anti-4-4-20 antibody) (circles) or 1B7 (ligand-inhibitable anti-4-4-20 antibody) (1 μg/ml) (open triangles). BiSCA was compared to mAb 04-01 (crosses; solid line) and mAb 4-4-20 (closed triangles) (1 μg/ml starting concentration) detected with protein A-HRP. B, anti-ssDNA activity of ssDNA-purified BiSCA (triangles) as compared to equal molar concentrations (1 x 10⁻⁷ M) mAb 04-01 (crosses; solid line). mAb 4-4-20 was hibition of mAb 04-01 and BiSCA binding used as a negative control (circles). C, into ssDNA by soluble DNA. Singlestranded DNA-coated polystyrene wells were incubated with equal molar concentrations(1 x 10⁻⁷ M) of BiSCA (crosses; solid line) and mAb 04-01 (triangles) after preincubation overnight with calf thymus DNA (0.5 mg/ml starting concentration). Individual points represent mean values of duplicate trials.
Mallender, W. D., & Voss, E. W. (1994). Construction, expression, and activity of a bivalent bispecific single-chain antibody. Journal of Biological Chemistry, 269(1), 199-206.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
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CAT | Product Name | Application | Type |
---|---|---|---|
MHH-922-F(E) | Recombinant Human Anti-ssDNA Antibody Fab Fragment | WB, FC, FuncS | Fab |
PFBZ-115 | Mouse Anti-ssDNA Recombinant Antibody (clone BV04-01); Fab Fragment | WB, ELISA | Mouse Fab |
PFBW-115 | Mouse Anti-ssDNA Recombinant Antibody (clone DNA-1); Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
FAMAB-0304CQ-F(E) | Mouse Anti-ssDNA Recombinant Antibody (clone BXW-DNA14); Fab Fragment | ELISA | Mouse Fab |
FAMAB-0305CQ-F(E) | Mouse Anti-ssDNA Recombinant Antibody (clone BXW-DNA16); Fab Fragment | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABW-115 | Mouse Anti-ssDNA Recombinant Antibody (clone DNA-1) | WB, IHC, IF, FuncS | Mouse IgG |
FAMAB-0059WJ | Human Anti-ssDNA Recombinant Antibody (clone 7B3) | ELISA, Inhib | Human IgM |
MOB-024YJL | Mouse Anti-ssDNA Recombinant Antibody (clone CB65YJ) | ELISA, FC, ICC, IHC-P | Mouse IgG3 |
FAMAB-0067JF | Mouse Anti-ssDNA Recombinant Antibody (clone 9F11) | ELISA, IHC, FC | Mouse IgG2b, κ |
FAMAB-0688WJ | Human Anti-ssDNA Recombinant Antibody (clone Kim11.4) | ELISA, WB | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0347MC | Recombinant Human Anti-ssDNA antibody | IHC, IP, FC |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0070WJ-S(P) | Human Anti-ssDNA Recombinant Antibody (clone 16/6); scFv Fragment | ELISA | Human scFv |
FAMAB-0110WJ-S(P) | Human Anti-ssDNA Recombinant Antibody (clone 2F7); scFv Fragment | ELISA, WB | Human scFv |
FAMAB-0111WJ-S(P) | Human Anti-ssDNA Recombinant Antibody (clone 1A6); scFv Fragment | ELISA, WB | Human scFv |
FAMAB-0068JF-S(P) | Mouse Anti-ssDNA Recombinant Antibody (clone 11F8); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
FAMAB-0325JF-S(P) | Mouse Anti-ssDNA Recombinant Antibody (clone dC1); scFv Fragment | ELISA, FuncS | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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