This product is a recombinant human antibody that recognizes TNFRSF8. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Figure 1 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in ELISA
ELISA analysis of HPAB-0333-YC was performed by coating with Human CD30/TNFRSF8 Protein (His Tag). Then blocked with BSA and incubated with HPAB-0333-YC. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 8000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.4 μg)
Lane 3: Reducing Antigen (0.6 μg)
Figure 2 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in WB
Western blot analysis of HPAB-0333-YC was performed with Human CD30/TNFRSF8 Protein (His Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0333-YC (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Figure 3 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in DB
Dot Blot analysis of HPAB-0333-YC was performed by coating with Human CD30/TNFRSF8 Protein (His Tag).
HPAB-0333-YC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-144 | Anti-Human CD30 Recombinant Antibody (Iratumumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-153 | Chimeric (Mouse/Human) Anti-TNFRSF8 Recombinant Antibody (clone Brentuximab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | Human IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G062E | Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-PE | Cytotoxicity assay, Function study | |
AGTO-G062R | Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-RTA | Cytotoxicity assay, Function study | |
AGTO-G062S | Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-Sap | Cytotoxicity assay, Function study | |
AGTO-L041E | anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-PE | Cytotoxicity assay, Functional assay | |
AGTO-L041R | anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-RTA | Cytotoxicity assay, Functional assay |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-635CL | Human Anti-TNFRSF8 Recombinant Antibody (TAB-635CL) | ELISA | Human IgG |
TAB-103LC | Anti-Human TNFRSF8 Recombinant Antibody | WB, FC, ELISA | Humanized antibody |
TAB-103LC-S(P) | Anti-Human TNFRSF8 Recombinant Antibody scFv Fragment | WB, FC, ELISA | Humanized antibody |
TAB-103LC-F(E) | Anti-Human TNFRSF8 Recombinant Antibody Fab Fragment | WB, FC, ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-139LC | Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-139LC-1 | Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0597MZ | Chicken Anti-TNFRSF8 Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-2165CQ | Hamster Anti-Tnfrsf8 Recombinant Antibody (clone mCD30.1) | FC, Stim, Costim, Block | Hamster IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-2166CQ | Hamster Anti-Tnfrsf8 Recombinant Antibody (clone 2SH12-5F) | Neut, FC | Hamster IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-3617 | Rabbit Anti-TNFRSF8 Recombinant Antibody (clone DS3617AB) | WB, IHC-P | Rabbit IgG |
MOR-3618 | Rabbit Anti-TNFRSF8 Recombinant Antibody (clone DS3618AB) | ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0329-YC-F(E) | Mouse Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0329-YC-F(E)) | ELISA, FC, Cyt, FuncS | Mouse Fab |
HPAB-0330-YC-F(E) | Human Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0330-YC-F(E)) | ELISA, FC, Cyt, FuncS | Human Fab |
HPAB-0331-YC-F(E) | Mouse Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0331-YC-F(E)) | ELISA, FC, Cyt, FuncS | Mouse Fab |
HPAB-0332-YC-F(E) | Human Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0332-YC-F(E)) | FC, Cyt | Human Fab |
HPAB-0333-YC-F(E) | Human Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0333-YC-F(E)) | FC, Cyt, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0445-CN | Mouse Anti-TNFRSF8 Recombinant Antibody (clone T105) | WB, ELISA, FC | Mouse IgG1 |
HPAB-0447-CN | Mouse Anti-TNFRSF8 Recombinant Antibody (clone T405) | WB, ELISA | Mouse IgG2b |
HPAB-0250-FY | Recombinant Human Anti-TNFRSF8 Antibody | F, IH | Human IgG1 |
HPAB-0459-WJ | Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0459-WJ) | ELISA, FuncS | Mouse IgG |
HPAB-1929-FY | Human Anti-TNFRSF8 Recombinant Antibody (clone 17G1) | ELISA, FC, ADCC | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-144 | Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody (Iratumumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
AFC-TAB-153 | Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody (Brentuximab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0459-WJ-S(P) | Mouse Anti-TNFRSF8 Recombinant Antibody; scFv Fragment (HPAB-0459-WJ-S(P)) | ELISA | Mouse scFv |
HPAB-1929-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 17G1); scFv Fragment | FC, ELISA | Human scFv |
HPAB-1930-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 2H9); scFv Fragment | FC, ELISA | Human scFv |
HPAB-1931-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 5F11); scFv Fragment | FC, ELISA | Human scFv |
FAMAB-0358WJ-S(P) | Human Anti-TNFRSF8 Recombinant Antibody (clone AK30); scFv Fragment | ELISA, WB | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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