Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) (CAT#: HPAB-0333-YC)

This product is a recombinant human antibody that recognizes TNFRSF8. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.


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ELISA

Figure 1 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in ELISA

Figure 1 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in ELISA

ELISA analysis of HPAB-0333-YC was performed by coating with Human CD30/TNFRSF8 Protein (His Tag). Then blocked with BSA and incubated with HPAB-0333-YC. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 8000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.4 μg)
Lane 3: Reducing Antigen (0.6 μg)

WB

Figure 2 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in WB

Figure 2 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in WB

Western blot analysis of HPAB-0333-YC was performed with Human CD30/TNFRSF8 Protein (His Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0333-YC (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.

DB

Figure 3 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in DB

Figure 3 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in DB

Dot Blot analysis of HPAB-0333-YC was performed by coating with Human CD30/TNFRSF8 Protein (His Tag).

HPAB-0333-YC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control


Specifications

  • Immunogen
  • Soluble CD30
  • Host Species
  • Human
  • Type
  • Human IgG, κ
  • Specificity
  • Human TNFRSF8
  • Species Reactivity
  • Human
  • Applications
  • FC, Cyt, FuncS
  • Related Disease
  • HD

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • See the COA
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • CD30; Ki-1; D1S166E

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "TNFRSF8"

Human Antibody

CAT Product Name Application Type
TAB-144 Anti-Human CD30 Recombinant Antibody (Iratumumab) ELISA, FC, IP, FuncS, IF, Neut, ICC IgG1 - kappa

Chimeric Antibody

CAT Product Name Application Type
TAB-153 Chimeric (Mouse/Human) Anti-TNFRSF8 Recombinant Antibody (clone Brentuximab) Neut, ELISA, IF, IP, FuncS, FC, ICC Human IgG1, κ

Immunotoxin

CAT Product Name Application Type
AGTO-G062E Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-PE Cytotoxicity assay, Function study
AGTO-G062R Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-RTA Cytotoxicity assay, Function study
AGTO-G062S Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-Sap Cytotoxicity assay, Function study
AGTO-L041E anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L041R anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-RTA Cytotoxicity assay, Functional assay

Humanized Antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-139LC Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) ELISA Human antibody

Low- or Non-fucosylated Oligosaccharide

CAT Product Name Application Type
Gly-139LC-1 Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0597MZ Chicken Anti-TNFRSF8 Polyclonal IgY WB Chicken antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-2165CQ Hamster Anti-Tnfrsf8 Recombinant Antibody (clone mCD30.1) FC, Stim, Costim, Block Hamster IgG1

Neutralizing Antibody

CAT Product Name Application Type
NEUT-2166CQ Hamster Anti-Tnfrsf8 Recombinant Antibody (clone 2SH12-5F) Neut, FC Hamster IgG1, κ

Rabbit Monoclonal Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-144 Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody (Iratumumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-153 Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody (Brentuximab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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