Rat Anti-TSLP Recombinant Antibody (TAB-195CQ) (CAT#: TAB-195CQ)

This product is a recombinant Rat antibody that recognizes TSLP (Thymic stromal lymphopoietin). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.


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SDS-PAGE

Figure 1 Rat Anti-TSLP Recombinant Antibody (TAB-195CQ) in SDS-PAGE

Figure 1 Rat Anti-TSLP Recombinant Antibody (TAB-195CQ) in SDS-PAGE

SDS-PAGE analysis of TAB-195CQ in β-mercaptoethanol-reduced (Lane R) and non-reduced (Lane N-R) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

DB

Figure 2 Rat Anti-TSLP Recombinant Antibody (TAB-195CQ) in DB

Figure 2 Rat Anti-TSLP Recombinant Antibody (TAB-195CQ) in DB

Dot Blot analysis of TAB-195CQ was performed by coating with human TSLP protein.

TAB-195CQ incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Rat IgG as a secondary antibody (1: 2000)

WB

Figure 3 Rat Anti-TSLP Recombinant Antibody (TAB-195CQ) in WB

Figure 3 Rat Anti-TSLP Recombinant Antibody (TAB-195CQ) in WB

Western blot analysis of TAB-195CQ was performed by loading human TSLP protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-195CQ and HRP goat anti-Rat IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

ELISA

Figure 4 Rat Anti-TSLP Recombinant Antibody (TAB-195CQ) in ELISA

Figure 4 Rat Anti-TSLP Recombinant Antibody (TAB-195CQ) in ELISA

ELISA analysis of TAB-195CQ was performed by coating with human TSLP protein. Then blocked with BSA and incubated with TAB-195CQ. The HRP-conjugated goat anti-Rat IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Immunogen
  • TSLP
  • Host Species
  • Rat
  • Type
  • Rat IgG1
  • Specificity
  • Human TSLP (Thymic stromal lymphopoietin)
  • Species Reactivity
  • Human, Cynomolgous monkey
  • Applications
  • Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Flow Cytometry; Neutralization
  • Related Disease
  • Inflammatory disorders

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • TSLP; Thymic stromal lymphopoietin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "TSLP"

Single-domain Antibody

CAT Product Name Application Type
NAB-2482-sdAb Recombinant Anti-Human TSLP VHH Single Domain Antibody ICC, ChiP, FC, FA Llama VHH

Rat Antibody

CAT Product Name Application Type
TAB-652CL Human Anti-TSLP Recombinant Antibody (TAB-652CL) FuncS Human IgG2λ

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-626CL Afuco™ Anti-TSLP Recombinant Antibody (AFC-626CL), ADCC Enhanced ELISA, WB Human IgG1, λ
AFC-TAB-454CQ Afuco™ Anti-TSLP Recombinant Antibody (AFC-TAB-454CQ), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS Human IgG1, λ

Humanized Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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