Mouse Anti-UPAR Recombinant Antibody (clone ATN615) (CAT#: PABC-130)
Recombinant Mouse Antibody (ATN615) is capable of binding to UPAR, expressed in Chinese Hamster Ovary cells (CHO). This antibody binds to uPAR in vitro with high affinity (K(d) approximately 1 nM) and does not interfere with uPA binding to uPAR.
Specific Inquiry
IP
Figure 1 Immunoprecipitation of AGM suPAR clones using ATN-615 and ATN-658.
A. Single point mutants were introduced into AGM suPAR such that a single amino acid was changed from the AGM sequence to the human uPAR sequence and the proteins expressed and purified as described in the Materials and Methods.
Xu, X. , Cai, Y. , Wei, Y. , Donate, F. , Juarez, J. , & Parry, G. , et al. (2014). Identification of a new epitope in upar as a target for the cancer therapeutic monoclonal antibody atn-658, a structural homolog of the upar binding integrin cd11b (αm). Plos One, 9(1), e98490.
ELISA
Figure 2 ELISA
ATN615 Fab binds to purified suPAR with high affinity. Binding of intact antibody ATN615 (○) or ATN615 Fab (●) to suPAR immobilized on plastic was determined using a modified ELISA assay. The affinity of ATN615 Fab for suPAR (0.84( ± 0.16) nM, n= 5) was indistinguishable from that of intact ATN615 antibody (0.94( ± 0.34) nM, n= 5). Results of a typical experiment are shown.
Li, Y., Parry, G., Chen, L., Callahan, J. A., Shaw, D. E., Meehan, E. J., ... & Huang, M. (2007). An anti-urokinase plasminogen activator receptor (uPAR) antibody: crystal structure and binding epitope. Journal of molecular biology, 365(4), 1117-1129.
Specifications
- Immunogen
- Human plasminogen activator, urokinase receptor
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human UPAR
- Species Reactivity
- Human
- Clone
- ATN615
- Applications
- ELISA, IP, WB, IHC, IF, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody ATN615 has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunoprecipitation, Western Blot, Immunohistochemistry, Immunofluorescence, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1024 | Recombinant Anti-uPAR Antibody | WB, IP, FuncS | IgG |
| MHH-1024 | Recombinant Human Anti-uPAR Antibody | ELISA, IP, FuncS | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1024-F(E) | Recombinant Anti-uPAR Antibody Fab Fragment | WB, IF, FuncS | Fab |
| MHH-1024-F(E) | Recombinant Human Anti-uPAR Antibody Fab Fragment | WB, FC, FuncS | Fab |
| PFBC-130 | Mouse Anti-UPAR Recombinant Antibody (clone ATN615); Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1024-S(P) | Recombinant Anti-uPAR Antibody scFv Fragment | FC, IP, FuncS | scFv |
| MHH-1024-S(P) | Recombinant Human Anti-uPAR Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| PSBC-130 | Mouse Anti-UPAR Recombinant Antibody (clone ATN615); scFv Fragment | WB, IHC, IF, FuncS | Mouse scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0392CL | Anti-Human UPAR Recombinant Antibody (10H6) | IHC, ELISA, Inhib | |
| TAB-0393CL | Anti-Human UPAR Recombinant Antibody (8B12) | SPR, Inhib | |
| TAB-0394CL | Anti-Human UPAR Recombinant Antibody (13D11) | IHC, ELISA, Inhib | |
| TAB-0395CL | Anti-Human UPAR Recombinant Antibody (19.1) | Inhib | |
| TAB-0396CL | Anti-Human UPAR Recombinant Antibody (AL6) | IHC, ELISA, Inhib |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0398CL | Anti-Human UPAR Recombinant Antibody (3B6) | IHC, ELISA, Inhib | |
| TAB-0399CL | Anti-Human UPAR Recombinant Antibody (3C10) | IHC, ELISA, Inhib | |
| TAB-0400CL | Anti-Human UPAR Recombinant Antibody (1C1) | IHC, ELISA, Inhib | |
| TAB-0401CL | Anti-Human UPAR Recombinant Antibody (2B10) | IHC, ELISA, Inhib | |
| TAB-0402CL | Anti-Human UPAR Recombinant Antibody (2A3) | IHC, ELISA, Inhib |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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