This monoclonal antibody, specific for urokinase-type plasminogen activator receptor (uPAR), can be used for the treatment or prevention of cancer.
Figure 1 Real binding kinetics of the uPAR·8B12 interaction as assessed by surface plasmon resonance.
The anti-uPAR mAb 8B12 was covalently immobilized on a CM5 sensor chip by amine chemistry to 610 RU (4.1 fmol/mm²). (a) The interaction with uPAR¹⁻²⁸³ was subsequently measured by 100 s injections of 2-fold serial dilutions of 100, 50, 25, 12, 6, and 3 nM purified receptor followed by 1800 s dissociation phases before regeneration. The blue sensorgrams show the repeat analyses of 50 and 25 nM uPAR, whereas the green line represents buffer injection. The red continuous lines represent the fit to the experimental data using a simple bimolecular interaction model including correction for mass transport limitations.
Zhao, B., Gandhi, S., Yuan, C., Luo, Z., Li, R., Gårdsvoll, H., ... & Ploug, M. (2015). Stabilizing a flexible interdomain hinge region harboring the SMB binding site drives uPAR into its closed conformation. Journal of molecular biology, 427(6), 1389-1403.
Figure 2 Inhibition of uPAR-dependent adhesion of HEK293 cells on vitronectin by mAbs from epitope bin 6.
The inhibitory effects of anti-uPAR mAbs (100 nM) belonging to epitope bin 6 (mAbs 8B12 and 19.10) and bin 1 (mAb R21) were compared on adherent HEK293 cells. The HEK293 cells were induced to adhere firmly on vitronectin by their surface expression of uPARwt, uPARwt·ATF complexes (formed by addition of 50 nM ATF), or uPARH47C-N259C. Cellular protrusions were scored 24 h after addition of the respective mAbs or buffer controls and each bar represents the mean value of three independent measurements (a protrusion index of 100% signifies that all 18 micrographs examined were scored positive for the presence of lamellipodia by both evaluators). Note the that the y-axis has been shifted upwards to allow the visualization of zero values.
Zhao, B., Gandhi, S., Yuan, C., Luo, Z., Li, R., Gårdsvoll, H., ... & Ploug, M. (2015). Stabilizing a flexible interdomain hinge region harboring the SMB binding site drives uPAR into its closed conformation. Journal of molecular biology, 427(6), 1389-1403.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1024 | Recombinant Anti-uPAR Antibody | WB, IP, FuncS | IgG |
MHH-1024 | Recombinant Human Anti-uPAR Antibody | ELISA, IP, FuncS | IgG |
PABC-130 | Mouse Anti-UPAR Recombinant Antibody (clone ATN615) | ELISA, IP, WB, IHC, IF, FuncS | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1024-F(E) | Recombinant Anti-uPAR Antibody Fab Fragment | WB, IF, FuncS | Fab |
MHH-1024-F(E) | Recombinant Human Anti-uPAR Antibody Fab Fragment | WB, FC, FuncS | Fab |
PFBC-130 | Mouse Anti-UPAR Recombinant Antibody (clone ATN615); Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1024-S(P) | Recombinant Anti-uPAR Antibody scFv Fragment | FC, IP, FuncS | scFv |
MHH-1024-S(P) | Recombinant Human Anti-uPAR Antibody scFv Fragment | FC, Neut, Funcs | scFv |
PSBC-130 | Mouse Anti-UPAR Recombinant Antibody (clone ATN615); scFv Fragment | WB, IHC, IF, FuncS | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0392CL-F(E) | Anti-Human UPAR Recombinant Antibody Fab Fragment (10H6) | IHC, ELISA, Inhib | |
TAB-0393CL-F(E) | Anti-Human UPAR Recombinant Antibody Fab Fragment (8B12) | IHC, ELISA, Inhib, In vivo | |
TAB-0394CL-F(E) | Anti-Human UPAR Recombinant Antibody Fab Fragment (13D11) | IHC, ELISA, Inhib | |
TAB-0395CL-F(E) | Anti-Human UPAR Recombinant Antibody Fab Fragment (19.1) | IHC, ELISA, Inhib | |
TAB-0396CL-F(E) | Anti-Human UPAR Recombinant Antibody Fab Fragment (AL6) | IHC, ELISA, Inhib |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0401CL-F(E) | Anti-Human UPAR Recombinant Antibody Fab Fragment (2B10) | IHC, ELISA, Inhib | |
TAB-0402CL-F(E) | Anti-Human UPAR Recombinant Antibody Fab Fragment (2A3) | IHC, ELISA, Inhib | |
TAB-0403CL-F(E) | Anti-Human UPAR Recombinant Antibody Fab Fragment (1G5) | IHC, ELISA, Inhib | |
TAB-0404CL-F(E) | Anti-Human UPAR Recombinant Antibody Fab Fragment (23D9) | ELISA, WB | |
TAB-0405CL-F(E) | Anti-Human UPAR Recombinant Antibody Fab Fragment (12H10) | ELISA, WB |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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