Recombinant Mouse Anti-VACV D8L Antibody (LA5) (CAT#: PABL-740)

Recombinant Mouse Antibody (LA5) is capable of binding to VACV D8L , expressed in Chinese Hamster Ovary cells (CHO). This antibody targets a discontinuous conformational epitope on D8.


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Figure 1 LA5 IgG specificity and neutralization activity.

Figure 1 LA5 IgG specificity and neutralization activity.

(A) MAb LA5 binds recombinant D8 by ELISA. An anti-H3 MAb (clone 41) was used as a negative control. (B) Peptide ELISA using overlapping 20-mer D8 peptides (peptides 1 to 35). Anti-D8 MAb LA5 showed reactivity to D8 but not to any of the overlapping D8 peptides, recombinant H3 (H), or a buffer-only control (0). (C) Surface plasmon resonance sensogram showing high-affinity binding (KD = 0.18 nM) of D8 to immobilized LA5 IgG. (D) In vitro VACV neutralization assays were done using anti-D8 MAb LA5 and anti-H3 MAb 41. Neutralization assays were done in the absence (left) or presence (middle) of complement (C′). VACV extracellular virions (EV) alone and plus complement were negative controls (right). Dashed lines indicate 50% neutralization. Data are representative of three or more independent experiments. Error bars indicate the standard error of the mean (SEM) in each group.

Matho, M. H. , Maybeno, M. , Benhnia, R. E. I. , Becker, D. , Meng, X. , & Xiang, Y. , et al. (2012). Structural and biochemical characterization of the vaccinia virus envelope protein d8 and its recognition by the antibody la5. Journal of Virology, 86(15), 8050-8058.


Specifications

  • Immunogen
  • VACV D8L; putative
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native VACV D8L
  • Species Reactivity
  • VACV
  • Clone
  • LA5
  • Applications
  • ELISA, Neut

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The VACV D8L antibody has been reported in applications of ELISA.
    ELISA: Plates were washed and incubated with purified MAb (LA5) at 10 ug/ml for 90 min at RT.

Target

  • Alternative Names
  • D8L; putative; VACVgp143

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Recombinant Antibody

CAT Product Name Application Type
MOB-1033 Recombinant Anti-VACV D8L Antibody ELISA, WB, IP, FuncS IgG
MHH-1033 Recombinant Human Anti-VACV D8L Antibody ELISA, WB, IF, FuncS IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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