Recombinant Mouse Antibody (LA5) is capable of binding to VACV D8L , expressed in Chinese Hamster Ovary cells (CHO). This antibody targets a discontinuous conformational epitope on D8.
Figure 1 LA5 IgG specificity and neutralization activity.
(A) MAb LA5 binds recombinant D8 by ELISA. An anti-H3 MAb (clone 41) was used as a negative control. (B) Peptide ELISA using overlapping 20-mer D8 peptides (peptides 1 to 35). Anti-D8 MAb LA5 showed reactivity to D8 but not to any of the overlapping D8 peptides, recombinant H3 (H), or a buffer-only control (0). (C) Surface plasmon resonance sensogram showing high-affinity binding (KD = 0.18 nM) of D8 to immobilized LA5 IgG. (D) In vitro VACV neutralization assays were done using anti-D8 MAb LA5 and anti-H3 MAb 41. Neutralization assays were done in the absence (left) or presence (middle) of complement (C′). VACV extracellular virions (EV) alone and plus complement were negative controls (right). Dashed lines indicate 50% neutralization. Data are representative of three or more independent experiments. Error bars indicate the standard error of the mean (SEM) in each group.
Matho, M. H. , Maybeno, M. , Benhnia, R. E. I. , Becker, D. , Meng, X. , & Xiang, Y. , et al. (2012). Structural and biochemical characterization of the vaccinia virus envelope protein d8 and its recognition by the antibody la5. Journal of Virology, 86(15), 8050-8058.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1033 | Recombinant Anti-VACV D8L Antibody | ELISA, WB, IP, FuncS | IgG |
MHH-1033 | Recombinant Human Anti-VACV D8L Antibody | ELISA, WB, IF, FuncS | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1033-F(E) | Recombinant Anti-VACV D8L Antibody Fab Fragment | Neut, IF, FuncS | Fab |
MHH-1033-F(E) | Recombinant Human Anti-VACV D8L Antibody Fab Fragment | WB, IHC, FuncS | Fab |
PFBL-733 | Recombinant Mouse Anti-VACV D8L Antibody Fab Fragment (LA5) | ELISA, Neut | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1033-S(P) | Recombinant Anti-VACV D8L Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
MHH-1033-S(P) | Recombinant Human Anti-VACV D8L Antibody scFv Fragment | WB, Neut, FuncS | scFv |
PSBL-733 | Recombinant Mouse Anti-VACV D8L Antibody scFv Fragment (LA5) | ELISA, Neut | scFv |
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