Recombinant Mouse Anti-fluorescein Antibody (4-4-20) (CAT#: PABX-037)

Figure 1 Kinetics of binding at 37 °C of 1 μM 4-4-20 IgG to 200 nM Hexaflutax bound to 20 μM paclitaxel binding sites in MAP-stabilized microtubules in AB (a control was performed to check that essentially all of the Hexaflutax was specifically bound to the microtubules).

Solid line, kinetics of binding at 37 °C of 1 μM 4-4-20 IgG to 200 nM Hexaflutax bound to 20 μM paclitaxel binding sites in MAP-stabilized microtubules in AB (a control was performed to check that essentially all of the Hexaflutax was specifically bound to the microtubules). Dotted line, kinetics of binding at 37 °C of 1 μM MOPC 21 IgG to 200 nM Hexaflutax bound to 20 μM paclitaxel binding sites in MAP-stabilized microtubules in AB. Data were corrected for the observed photobleaching (5%). Inset, dependence of the observed kinetic rate on the concentration of IgG.

Díaz, J. F., Barasoain, I., Souto, A. A., Amat-Guerri, F., & Andreu, J. M. (2005). Macromolecular accessibility of fluorescent taxoids bound at a paclitaxel binding site in the microtubule surface. Journal of Biological Chemistry, 280(5), 3928-3937.