Human Anti-HIV gp41 Recombinant Antibody (clone Z13e1) (CAT#: PABX-064)
Anti-gp41 antibody is a Human antibody of IgG1 λ class that binds to an gp41. This antibody binds the epitope (W (670)NWFDITN (677)).
Specific Inquiry
Neut
Figure 1 Neutralization of pseudotyped HIV-1MN by Fab Z13, Fab Z13e1, and 4E10 IgG in U87.CD4.CXCR4 target cells in a single-cycle infectivity assay.
The ∼100-fold improvement in the neutralization potency of Fab Z13e1 relative to that of Fab Z13 is indicated at the level of IC₈₀.
Nelson, J. D., Brunel, F. M., Jensen, R., Crooks, E. T., Cardoso, R. M., Wang, M., ... & Burton, D. R. (2007). An affinity-enhanced neutralizing antibody against the membrane-proximal external region of human immunodeficiency virus type 1 gp41 recognizes an epitope between those of 2F5 and 4E10. Journal of virology, 81(8), 4033-4043.
ELISA
Figure 2 Competition ELISA using IgGs Z13e1, 2F5, and 4E10 against their biotinylated (Bio) counterparts for recognition of immobilized M41xt (MBP fused to gp41JRFL ectodomain; aa 535 to 681).
Biotinylated IgG Z13e1 (left panel), biotinylated IgG 4E10 (middle panel), and biotinylated IgG 2F5 (right panel) were added to wells that were coated with immobilized M41xt and pretreated with various concentrations of competing MAb. Each data point represents the mean of duplicate samples.
Nelson, J. D., Brunel, F. M., Jensen, R., Crooks, E. T., Cardoso, R. M., Wang, M., ... & Burton, D. R. (2007). An affinity-enhanced neutralizing antibody against the membrane-proximal external region of human immunodeficiency virus type 1 gp41 recognizes an epitope between those of 2F5 and 4E10. Journal of virology, 81(8), 4033-4043.
WB
Figure 3 10E8 binds less efficiently to Env spikes of MPER mutant F673A compared to wild type.
(E) 10E8, 4E10 or Z13e1 Fabs were incubated with JR-FL wild type or F673A virions and resulting changes to gel mobility of Env trimers was determined using BN-PAGE and Western blot. A cocktail of gp120 and gp41 antibodies were used to probe the blot. Electrophoresis was performed using 3–8% Tris-Acetate NuPAGE gels (Invitrogen) under native conditions. Stoichiometry of binding was determined by measuring the gel mobility shift compared to Fab controls b12 and PG9.
Kim, A. S., Leaman, D. P., & Zwick, M. B. (2014). Antibody to gp41 MPER alters functional properties of HIV-1 Env without complete neutralization. PLoS pathogens, 10(7), e1004271.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Type
- Human IgG1, λ
- Specificity
- Tested positive against HIV-1 gp41
- Species Reactivity
- HIV-1
- Clone
- Z13e1
- Applications
- WB, Neut, FuncS, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV gp41 antibody has been reported in applications of Western Blot, Neutralization, Functional Assay, Neutralization, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- HIV gp41; anti-HIV gp41
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-471 | Recombinant Anti-HIV gp41 Antibody | ELISA, IP, FuncS | IgG |
| MHH-471 | Recombinant Human Anti-HIV gp41 Antibody | RIA, WB, FuncS | IgG |
| PABL-139 | Human Anti-HIV gp41 (596-606) Recombinant Antibody (clone 7B2) | Neut, FC, FuncS | Human IgG1, κ |
| HPAB-1987-FY | Human Anti-HIV gp41 Recombinant Antibody (clone Z13) | ELISA, FC | Human IgG, κ |
| FAMAB-0099WJ | Human Anti-HIV gp41 Recombinant Antibody (clone 120-16) | ELISA | Human IgG2 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-471-F(E) | Recombinant Anti-HIV gp41 Antibody Fab Fragment | RIA, WB, FuncS | Fab |
| MHH-471-F(E) | Recombinant Human Anti-HIV gp41 Antibody Fab Fragment | IP, IF, Biosensors, FuncS | Fab |
| PFBL-139 | Human Anti-HIV gp41 (596-606) Recombinant Antibody (clone 7B2); Fab Fragment | Neut, FC, FuncS | Human Fab |
| HPAB-1987-FY-F(E) | Human Anti-HIV gp41 Recombinant Antibody (clone Z13); Fab Fragment | FC, Neut | Human Fab, κ |
| FAMAB-0099WJ-F(E) | Human Anti-HIV gp41 Recombinant Antibody (clone 120-16); Fab Fragment | ELISA | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-471-S(P) | Recombinant Human Anti-HIV gp41 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| PSBL-139 | Human Anti-HIV gp41 (596-606) Recombinant Antibody (clone 7B2); scFv Fragment | Neut, FC, FuncS | Human scFv |
| HPAB-1987-FY-S(P) | Human Anti-HIV gp41 Recombinant Antibody (clone Z13); scFv Fragment | FC, Neut | Human scFv |
| FAMAB-0082WJ-S(P) | Human Anti-HIV gp41 Recombinant Antibody (clone NO.86); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0262JF-S(P) | Monkey Anti-HIV gp41 Recombinant Antibody (clone B402); scFv Fragment | FC, ELISA, FuncS | Monkey scFv |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-092CT-S(P) | Human Anti-HIV gp41 Recombinant Antibody; scFv Fragment (TAB-092CT-S(P)) | ELISA, Neut | Human scFv |
| PABX-064-S (P) | Recombinant Human Anti-gp41 Antibody scFv Fragment (Z13e1) | WB, Neut, FuncS | scFv |
| PABX-064-F (E) | Recombinant Human Anti-gp41 Antibody Fab Fragment (Z13e1) | WB, Neut, FuncS | Fab |
| PABX-064-F(E) | Human Anti-HIV gp41 Recombinant Antibody (clone Z13e1); Fab Fragment | WB, Neut, FuncS, ELISA | Human Fab |
| PABX-064-S(P) | Human Anti-HIV gp41 Recombinant Antibody (clone Z13e1); scFv Fragment | WB, Neut, FuncS, ELISA | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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