Recombinant Chicken Antibody (pT231/pS235_1) is capable of binding to phospho-tau. This antibody exhibits a KD of 0.35 nm in 1:1 binding to its cognate phosphopeptide.
Figure 1 Expression and binding activity of chimeric IgG molecules converted from scFv.
Western blot analysis of chimeric IgG molecules converted from scFv clones pT231/pS235_1, pS396/pS404_1, and pT212/pS214_1, respectively. The heavy and light chains for each IgG are indicated by arrows .
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
Figure 2 Expression and binding activity of chimeric IgG molecules converted from scFv.
ELISA analysis of each purified IgG molecule for binding to the phosphopeptide, non-phosphopeptide, and scrambled ( Scr ) peptide.
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
Figure 3 Binding of pathological phospho-tau by antibody pT231/pS235_1 in human AD and Tg4510 transgenic mouse brains.
A – C , immunohisto- chemical staining of human AD brain ( A and B ) and healthy brain ( C ). B , magnification of the boxed area in A . D , Western blot analysis of Tg4510 transgenic and wild-type brain lysates with pT231/pS235_1 IgG (plus control anti-GAPDH antibody). Each lane represents a different animal, and the lysate samples are 3-month-old wild-type mice ( lanes 1 and 2 ), 3-month-old transgenic mice ( lanes 3 and 4 ), 6-month-old wild-type mice ( lanes 5 and 6 ), and 6-month-old transgenic mice ( lanes 7 and 8 ).
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1215z | Mouse Anti-MAPT Recombinant Antibody (clone 44C4) | ICC, IF, IHC, WB | Mouse IgG1 |
PABL-305 | Chicken Anti-MAPT Recombinant Antibody | WB, ELISA, FuncS | Chicken IgY |
PABZ-196 | Human Anti-MAPT Recombinant Antibody (clone pT231/pS235_1) | ELISA, WB | Chimeric (chicken/human) IgG |
HPAB-0082-FY | Mouse Anti-MAPT Recombinant Antibody (HPAB-0082-FY) | WB, ELISA | Mouse IgG |
HPAB-672-FY | Mouse Anti-MAPT Recombinant Antibody (HPAB-672-FY) | WB, ELISA | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0733MZ | Chicken Anti-TAU Polyclonal IgY | ICC/IF, IHC, WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-174 | Recombinant Mouse Anti-Tau5 Antibody | WB, ELISA, Neut, FuncS | IgG |
PABX-174-S (P) | Recombinant Mouse Anti-Tau5 Antibody scFv Fragment | WB, ELISA, Neut, FuncS | scFv |
PABX-174-F (E) | Recombinant Mouse Anti-Tau5 Antibody Fab Fragment | WB, ELISA, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2164 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (DS2164AB) | IHC-P | IgG |
MOR-4513 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH21DS) | WB, IHC-P, FC | IgG |
MOR-4714 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH228DS) | WB, IF, ICC, IHC-P, FC | IgG |
MOR-4715 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH229DS) | WB, IF, ICC, IHC-P | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-090CN | Mouse Anti-MAPT Recombinant Antibody (NS-090CN) | WB, ELISA, IHC, IP, SPR, FuncS | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0196-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-0196-YJ-F(E)) | ELISA | Mouse Fab |
HPAB-0197-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-0197-YJ-F(E)) | ELISA | Mouse Fab |
HPAB-0198-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody (clone 13A6); Fab Fragment | IA, IP | Mouse Fab |
HPAB-0199-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody (clone 10H8); Fab Fragment | ELISA, Quantification | Mouse Fab |
HPAB-0200-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody (clone 19G10); Fab Fragment | ELISA, Quantification | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0573-WJ-S(P) | Mouse Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-0573-WJ-S(P)) | ELISA | Mouse scFv |
HPAB-0574-WJ-S(P) | Mouse Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-0574-WJ-S(P)) | ELISA | Mouse scFv |
HPAB-1003WJ-S(P) | Human Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-1003WJ-S(P)) | ELISA, WB | Humanized scFv |
HPAB-0654YY-S(P) | Human Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-0654YY-S(P)) | Block, IHC | Humanized scFv |
HPAB-0655YY-S(P) | Human Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-0655YY-S(P)) | Block, IHC | Humanized scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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