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  • BV421-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer

BV421-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer (CAT#: MHC-LC844)

This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of ENO1-derived peptide of TSKGLFRAAVPSGAS covering 26-40 and DRB1*04:01 molecule. The pMHC tetramer recognizes CD4 T cells, and can be used in the analysis of individual antigen-specific T cells.


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  • Gene Expression
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
Figure 3 IF staining of human cell line A549
Figure 3 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
Figure 4 IHC staining of human pancreas
Figure 4 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
Figure 5 IHC staining of human kidney
Figure 5 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows moderate positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
Figure 6 IHC staining of human kidney
Figure 6 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
Figure 7 IF staining of human cell line MCF7
Figure 7 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Figure 8 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
Figure 9 Colon
Figure 9 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
Figure 10 Liver
Figure 10 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
Figure 11 Pancreas
Figure 11 Pancreas
(Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
Figure 12 Kidney
Figure 12 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
Figure 13 Testis
Figure 13 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
Figure 14 Lymph node
Figure 14 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
RNA Expression
Figure 15 RNA cell line category: Low cell line specificity
Figure 15 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1

Specifications

  • Allele
  • DRB1*04:01
  • Class
  • Class II
  • Antigen
  • ENO1
  • Antigen Species
  • Human
  • Peptide
  • TSKGLFRAAVPSGAS
  • Range
  • 26-40
  • Conjugate
  • BV421
  • Application
  • FCM

Target

  • Antigen Introduction
  • This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
  • Alternative Names
  • ENO1; enolase 1; NNE; PPH; MPB1; ENO1L1; HEL-S-17

Downloads

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See other products for "ENO1"

Human Antibody

CAT Product Name Application Type
TAB-350MZ Anti-Human ENO1 Recombinant Antibody (A3) WB, FC Human antibody
TAB-350MZ-S(P) Anti-Human ENO1 Recombinant Antibody scFv Fragment (A3) WB, FC Human antibody
TAB-350MZ-F(E) Anti-Human ENO1 Recombinant Antibody Fab Fragment (A3) WB, FC Human antibody

Recombinant Antibody

CAT Product Name Application Type
MOB-0661CT Recombinant Mouse anti-Human ENO1 Monoclonal antibody (3H3BH22CG9) FC, ICC/IF, IP, ELISA
HPAB-0054-CN Mouse Anti-ENO1 Recombinant Antibody (HPAB-0054-CN) FC, ELISA Mouse IgG
HPAB-A1275-YJ Chicken Anti-ENO1 Recombinant Antibody (clone EnL1) WB, ELISA Chicken IgG
HPAB-A1276-YJ Chicken Anti-ENO1 Recombinant Antibody (clone EnL4) WB, ELISA Chicken IgG
VS-0923-FY53 Recombinant Mouse Anti-ENO1 Antibody (VS-0923-FY53) ELISA, WB Mouse IgG1

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0190MZ Chicken Anti-ENO1 Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC842 PE-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer FCM
MHC-LC843 APC-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer FCM

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1151 Hi-Affi™ Rabbit Anti-ENO1 Recombinant Antibody (clone DS1151AB) WB, ICC, IF, IP Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0054-CN-S(P) Mouse Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0054-CN-S(P)) FC, ELISA Mouse scFv
HPAB-0055-CN-S(P) Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0055-CN-S(P)) FC, ELISA Humanized scFv
HPAB-J0072-YC-S(P) Human Anti-ENO1 Recombinant Antibody (clone B3); scFv Fragment ELISA Human scFv
HPAB-J0073-YC-S(P) Human Anti-ENO1 Recombinant Antibody (clone C4); scFv Fragment ELISA Human scFv
HPAB-J0074-YC-S(P) Human Anti-ENO1 Recombinant Antibody (clone B8); scFv Fragment ELISA Human scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0054-CN-F(E) Mouse Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0054-CN-F(E)) FC, ELISA Mouse Fab
HPAB-0055-CN-F(E) Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0055-CN-F(E)) FC, ELISA Humanized Fab
HPAB-0056-CN-F(E) Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0056-CN-F(E)) FC, ELISA Humanized Fab
FAMAB-0235-CN-F(E) Chicken Anti-ENO1 Recombinant Antibody (clone EnL2); Fab Fragment ELISA, WB, IF, FC Chicken Fab
FAMAB-0236-CN-F(E) Chicken Anti-ENO1 Recombinant Antibody (clone EnL5); Fab Fragment ELISA, WB, IF, FC Chicken Fab

MHC Tetramer for Other Applications

CAT Product Name Application Type
MHC-YF036 DRB1*04:01/Human cit alpha-enolase (IFDS[cit]GNPTVEVDLF) MHC Monomer MHC Multimer

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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