Chymotrypsin Protease (TLCK treated), MS Grade (CAT#: Glyco-078CL)
Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
Specific Inquiry
Specifications
- Product Size
- 4 x 25 µg
- Final Product
- Peptide
- Workflow Step
- Protein Digestion
- Detection Method
- Mass Spectrometry
- Starting Material
- Cell Lysate, Protein samples
- For Use With (Equipment)
- Mass Spectrometer
- Contents & storage
- Store at -20°C in a nonfrost-free freezer.
Background
- Description
- This Chymotrypsin is a mass spectrometry (MS)-grade serine protease isolated from bovine pancreatic extracts. This chymotrypsin is TLCK-treated to eliminate tryptic activity. The selectivity of this enzyme is important for reproducible protein digestion and mass spectrometry-based protein identification. Chymotrypsin-digestion typically generates a larger number of shorter peptides compared to trypsin. This mass spectrometry-grade Chymotrypsin is packaged lyophilized (convenient 4 x 25 µg).
The endoproteinase chymotrypsin specifically cleaves at the carboxyl side of tyrosine, phenylalanine, tryptophan and leucine. Two predominant forms of chymotrypsin, A and B, are found in equal amounts in bovine pancreas. They are similar proteins (80% homology), but have different proteolytic characteristics. Both forms of chymotrypsin are present in Chymotrypsin. Since trypsin may co-purify with Chymotrypsin derived from natural sources, Chymotrypsin has been treated with TLCK to eliminate potential tryptic activity, improving digestion specificity. Chymotrypsin can tolerate mild denaturing conditions, such as 0.1% SDS, 2M urea, 2M guanidine·HCl, 1% CHAPS, and 30% acetonitrile with optimal activity in the pH range 7.5 to 8.5. This lyophilized enzyme has a mass of 25 kDa and is stable for 1 year when stored at -20°C.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for ""
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0402ZL | Human IgG1, kappa - Isotype Control Antibody | ELISA | |
| MRO-075-PMZ | Anti-C. difficile cell surface protein Polyclonal Antibody | WB, ELISA | Rabbit antibody |
| MRO-523-PMZ | Anti-Bacterial alkaline phosphatase Polyclonal Antibody (HRP) | ELISA | Rabbit antibody |
| MRO-623-RMZ | Recombinant Anti-P. aeruginosa Antibody (B11) | ELISA | Mouse antibody |
| MRO-776-RMZ | Recombinant Anti-Streptococcus equi subsp. equi Antibody (MAB212P) | ELISA | Mouse antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-171 | Recombinant Mouse Anti-Shigella flexneri variant Y Antibody (SYA/J6) | WB, ELISA, FuncS | IgG |
| PABX-171-S (P) | Recombinant Mouse Anti-Shigella flexneri variant Y Antibody scFv Fragment (SYA/J6) | WB, ELISA, FuncS | scFv |
| PABX-171-F (E) | Recombinant Mouse Anti-Shigella flexneri variant Y Antibody Fab Fragment (SYA/J6) | WB, ELISA, FuncS | Fab |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3377 | PE-B*14:02/Human (DRYRRIHSL) MHC Tetramer | FCM |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for Glyco-078CL. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.