Products

CBGlyco™ Direct IP Kit (Glyco-038CL)

Direct IP Kit uses an activated resin to covalently immobilize IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.

The primary benefits resulting from this method are the opportunity to use any species or subclass of purified antibody (not just types that bind to Protein A or G) and the ability to purify target protein without contamination by the antibody. The method also makes it possible to immunoprecipitate antigens from serum samples without co-purifying non-target immunoglobulins. Finally, the kit uses microcentrifuge spin cups to effectively wash and separate samples from the beaded agarose resin.      

Specifications
Product Size
50 reactions
Technique
Immunoprecipitation (IP), Pull-Down
Assay Type
Affinity Purification
Target Specificity
Not Target-Specific
For Use With (Equipment)
Microcentrifuge
Contents & storage
Sufficient For: Preparing and performing at least 50 IP reactions, each with 10 µL of resin
• Aminolink Plus Coupling Resin, 2 mL
• Coupling Buffer (20X), 25 mL
• Quenching Buffer, 50 mL
• Wash Solution, 50 mL
• Sodium Cyanoborohydride Solution (5M), 0.5 mL
• IP Lysis/Wash Buffer, 2 x 50 mL
• Conditioning Buffer (100X), 5 mL
• Tris-Buffered Saline (20X), 25 mL
• Elution Buffer, 50 mL
• Lane Marker Sample Buffer (5X), 5 mL
• Control Agarose Resin, 2 mL
• Spin Columns and Collection Tubes

Store at 4°C.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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