Products

Glu-C Protease, MS Grade (Glyco-079CL)

Glu-C Protease, MS Grade, is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.      

Specifications
Product Size
5 x 10 µg
Final Product
Peptide
Workflow Step
Protein Digestion
Detection Method
Mass Spectrometry
Starting Material
Cell Lysate, Protein samples
For Use With (Equipment)
Mass Spectrometer
Contents & storage
Store at -20°C in a nonfrost-free freezer.
BACKGROUND
Description
This Glu-C is a mass spectrometry (MS)-grade serine protease isolated from Staphylococcus aureus. Glu-C can be used alone or alongside trypsin or other proteases to produce complementary protein digests for peptide mapping and protein sequencing. Glu-C protease is suitable for either in-solution or in-gel digestion workflows. This Glu-C enzyme is packaged lyophilized (5 x 10 µg).

The endoproteinase Glu-C, also referred to as V-8 Protease, specifically cleaves the carboxyl side of glutamic residues when reactions are carried out in ammonium bicarbonate and ammonium acetate buffers, generating a limited number of peptide fragments. Cleavage can also occur at both glutamic and aspartic residues in phosphate buffers. Glu-C can efficiently digest protein in 5-18 hours at 37°C. Glu-C Protease remains active under denaturing conditions such as 2M urea, 1M guanidine·HCl, 0.1% SDS, 2% CHAPS and 20% acetonitrile. Glu-C activity is optimal at pH 8. This lyophilized enzyme has a mass of 27 kDa and is stable for 1 year when stored at -20°C.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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