Products

CBGlyco™ Glycoprotein Carbohydrate Estimation Kit (Glyco-059CL)

Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass.      

Specifications
Product Size
500 mg
Detection Method
Colorimetric
Technique
Absorbance, Solution-based Detection
Assay Type
Protein Quantitation Assay
For Use With (Equipment)
Microplate Reader, Spectrophotometer
Contents & storage
Sufficient For: 60 tube assays or 250 microplate assays
• Sodium meta-Periodate, 500 mg
• Glycoprotein Detection Reagent, 500 mg
• Glycoprotein Assay Buffer, 250 mL
• Glycoprotein Standards Set, 5-protein set
BACKGROUND
Description
The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (percent carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550 nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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