Products

CBGlyco™ Glycoprotein Staining Kit (Glyco-057CL)

Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.      

Specifications
Product Size
750 mL
Target Molecule
Proteins (Glycoproteins)
Label or Dye
Fuchsin Sulfite
Detection Method
Colorimetric
Detection Location
In-Gel Detection, In-Blot Detection
Contents & storage
Sufficient For: 10 mini gels (SDS-PAGE)
• Oxidation Reagent, 2.5 g powder to make 250 mL
• Reduction Reagent, 1.25 g powder to make 250 mL
• Positive Control (Horseradish Peroxidase), 1 mg
• Negative Control (Soybean Trypsin Inhibitor), 1 mg
• Glycoprotein Stain, 250 mL

Store all components at 4°C.
BACKGROUND
Description
The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

To use the Glycoprotein Staining Kit, simply treat the gel or membrane containing separated proteins treated with the provided periodate-based Oxidation Reagent. This treatment oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are reacted with the Glycoprotein Stain Reagent to form Schiff-base bonds that result in the production of magenta bands of the stain. Traditionally called the periodate-acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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