CBGlyco™ HRV 3C Protease Solution Kit (CAT#: Glyco-065CL)
Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
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Specifications
- Product Size
- 1,000 units
10,000 units
- Format
- Kit
- Contents & storage
- Store at -20°C.
Background
- Description
- HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.
- Properties
- • Alternative names: Human Rhinovirus 3C
• Source: Escherichia coli (E.coli)
• Molecular weight: 47.8kD
• Form: Liquid
• Protease type: Cysteine
• Optimal reaction conditions: 50 mM Tris-HCl, 150 mM NaCl, pH 8.0
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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