CBGlyco™ Hydrazide-PEG4-Biotin (CAT#: Glyco-027CL)
Hydrazide-PEG4-Biotin (also called Biotin-PEG4-Hydrazide) is an aldehyde-reactive biotinylation reagent that contains a 4-unit polyethylene glycol (PEG) spacer arm for increased hydrophilicity.
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Specifications
- Product Size
- 50 mg
- Label or Dye
- Biotin
- Spacer
- Pegylated, Extra-Long
- Solubility
- DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
- Cell Permeability
- Cell-Impermeant
- Chemical Reactivity
- Carboxylic Acid, Ketone, Aldehyde
- Contents & storage
- Store at 4°C.
Background
- Description
- Biotin-PEG4-Hydrazide enables biotinylation of glycoproteins and other molecules containing aldehydes or oxidizable sugar groups. The hydrazide group reacts spontaneously with aldehyde or ketone groups to form a stable hydrazone bond. Aldehyde groups can be created by gentle periodate oxidation of cis-diols in sialic acid and other sugars in carbohydrates. The hydrophilic, 4-unit polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG segment also adds length and flexibility to the spacer arm, minimizing steric hindrance involved with binding to avidin molecules.
- Properties
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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