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IMDM Media for SILAC (Glyco-095CL)

IMDM Media for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).      

Specifications
Product Size
500 mL
Workflow Step
Protein Labeling
Starting Material
Cells
For Use With (Equipment)
Mass Spectrometer
Contents & storage
Store in refrigerator (2 to 8°C).
BACKGROUND
Description
Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of "heavy" 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

This SILAC media is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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