Products

CBGlyco™ Immobilization Kit (Glyco-029CL)

Immobilization Kit contains affinity columns and reagents to immobilize glycoproteins through oxidized sugar groups for use in affinity-purification applications or studies of protein-protein interactions.      

Specifications
Product Size
10 columns
Format
Spin Column, Kit, 15 mL conical
Column Type
Affinity, UltraLink Resin
Immobilized Phase
Hydrazide
Contents & storage
Sufficient For: Immobilizing 1 to 10 mg of glycoprotein per column
• Columns, 1 mL, 10 columns
• Coupling Buffer, 2 x 100 mL
• Aniline, 0.2 mL
• Wash Buffer, 120 mL
• Sodium meta-periodate, 0.5 g
• Zeba Spin Desalting Columns, 5 mL, 10 columns
• PBS Pack (makes 500mL), 1 pack
• Column Accessories, 10 ea

Store at 4°C.
BACKGROUND
Description
The Kits use hydrazide-activated resin and a special catalyst to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized with these kits to make reusable affinity columns. Antibodies immobilized by this hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability. Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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