Jacalin Agarose (CAT#: Glyco-061CL)
Jacalin Agarose provides for specific affinity purification of human IgA from serum or colostrum or efficient removal of contaminating IgA from IgG samples.
Specific Inquiry
Specifications
- Product Size
- 5 mL
- Format
- Bottle(s)
- Column Type
- Affinity, Agarose Resin
- Immobilized Phase
- Lectin
- Contents & storage
- Upon receipt store product at 4°C. Product is shipped at ambient temperature. Do not freeze product.
Background
- Description
- Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilized on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.
Binding of IgA toimmobilized jacalin occurs at physiologic pH and ionic strength, as inphosphate-buffered saline (PBS). Elution of bound IgA occurs withcompetitor ligand (e.g., 0.1M melibiose or 0.1 M alpha-D-galactose) inPBS. IgD is reported to bind to jacalin (Aucouturier, et al., 1987).
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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