Products

CBGlyco™ Lumio Green Detection Kit (Glyco-054CL)

The Lumio Green Detection Kit is a sensitive and highly specific kit for labeling Lumio fusion proteins prior to electrophoresis. The Lumio Green Kit enables immediate visualization of Lumio fusion protein bands directly in a polyacrylamide gel.      

Specifications
Product Size
1 kit
Target Molecule
Proteins (Lumio-Tagged) , Proteins (TC-Tagged)
Label or Dye
Lumio Green
Detection Method
Fluorescent
Detection Location
In-Gel Detection
Contents & storage
The Lumio Green Detection Kit contains one vial of Lumio Green Detection Reagent, five vials of Lumio Gel Sample Buffer, and one vial of Lumio In-Gel Detection Enhancer.
Sufficient reagents are included in the kit for 100 in-gel detections or 20 in vitro transcription-translation labeling reactions.
Store at -20°C. All components are guaranteed stable for 6 months when properly stored.
BACKGROUND
Description
• Label proteins fused to a Lumio sequence
• Visualize results immediately after electrophoresis—eliminate gel staining and western blotting
• Detect nanogram levels of protein with a UV transilluminator, equipped with a standard camera, or a laser-based scanner
The Lumio Green Detection Reagent, included in the Lumio Green Detection Kit, can also be used for real-time detection of protein synthesis during in vitro transcription-translation reactions.
To detect using the Lumio Green Detection Kit simply:
1. Add the Lumio Green Detection Reagent and the optimized Lumio Sample Buffer to your protein sample and heat for 10 minutes at 70°C.
2. Add the Lumio In-Gel Detection Enhancer to your sample mix and incubate for 5 minutes at room temperature.
3. Load and electrophorese your protein gel.
4. Visualize your Lumio fusion protein bands with a UV transilluminator, equipped with a standard camera, or laser-based scanner.
5. After documenting your Lumio Green signal, total protein staining can be performed.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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