MEM Media for SILAC (CAT#: Glyco-098CL)

MEM Media for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).


Specific Inquiry
  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 500 mL
  • Workflow Step
  • Protein Labeling
  • Starting Material
  • Cells
  • For Use With (Equipment)
  • Mass Spectrometer
  • Contents & storage
  • Store in refrigerator (2 to 8°C).

Background

  • Description
  • Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of "heavy" 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

    This SILAC media is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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