Products

CBGlyco™ Maleimide-activated HRP (sulfhydryl-reactive) (Glyco-035CL)

Maleimide Activated Horseradish Peroxidase is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines. This product contains 5 mg of conjugated protein and can effectively modify 5 mg of immunoglobulin.      

Specifications
Product Size
5 mg
Label or Dye
HRP (Horseradish Peroxidase)
Solubility
Water
Chemical Reactivity
Thiol
Contents & storage
Note: Total weight is ~25mg as a result of buffer components and salts lyophilized along with the activated protein

Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.
BACKGROUND
Description
This product consists of horseradish peroxidase (HRP) that has been modified with Sulfo-SMCC to attach several maleimide groups per HRP molecule while retaining the peroxidase activity. The activated HRP will covalently attach to proteins or other molecule containing sulfhydryl groups (e.g., cysteines). HRP-conjugates of antibodies, proteins, peptides and other thiol-containing reporter probes are easily made using this method. The complete kit includes the activated HRP as well as two types of reagents for preparing sulfhydryl-ready antibodies (IgG) or proteins for conjugation.

The complete kit for Maleimide Activated Horseradish Peroxidase contains reagents for exposing or added the necessary sulfhydryl groups on antibodies (IgG) or practically any other protein. These general strategies are described briefly in the applications section of our review of Maleimide Reaction Chemistry. Of course, any protein that contains cysteines has sulfhydryl groups (-SH), but they must be reduced (not in the form of disulfide bonds) to be conjugated. Antibodies also contain disulfide bonds that can be targeted as antibody labeling sites; the hinge-region disulfide bonds in IgG can be selectively cleaved with the mild reducing agent 2-Mercaptoethylamine, which is included in the complete kit. Alternatively, sulfhydryl groups can be added to proteins (or any amine-containing molecule) using SATA reagent, which also is included in the kit.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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