Products

CBGlyco™ O-GlcNAc Western Blot Detection Kit (Glyco-058CL)

O-GlcNAc Western Blot Detection Kit contains a specific antibody for the O-GlcNAc post-translational modification (PTM), as well as detection reagents needed to perform a complete detection experiment.      

Specifications
Product Size
255 mL
Substrate
Luminol
Reactivity
All species
Detection Method
Western Blot
Specifications
Glycosylation and phosphorylation are two of the most extensively studied post-translational modifications because of their important role in the signaling pathways that regulate cellular response. The O-glycosidically linked modifications of serine or threonine residues are found on proteins from essentially all eukaryotic cells. Proteins in both the cytoplasm and nucleus can be found with this specific posttranslational modification. Although not precisely known, the function of the β-O-linked N-acetylglucosamine (O-GlcNAc) modification may serve as an alternative to phosphorylation, as a modulator of protein interactions in multimeric complexes or as a fortification against proteolytic attack.

The O-GlcNAc modification is seemingly related to phosphorylation in many respects. Notably, the modification is controlled similarly to that of phosphorylation. All O-GlcNAc-modified proteins currently known are also phosphoproteins. In addition, there is an apparent reciprocal relationship between these two important posttranslational modifications within a single protein. Therefore, there is considerable interest in the O-GlcNAc modification.
Contents & storage
• M-PER Mammalian Protein Extraction Reagent, 25 mL, store at room temperature
• Dilution Buffer (also functions as Blocking Buffer) (1X), 2 × 5 mL, store at 4°C
• BupH Phosphate Buffered Saline, 17 packs, store at room temperature
• Surfact-Amps 2 (1% Tween-2 Detergent), 3 × 1 mL, store at room temperature
• Goat Anti-Mouse IgM (µ), HRP Conjugate, 75 µg, store at 4°C
• SuperSignal West Dura Luminol/Enhancer Solution, 5 mL, store at room temperature
• SuperSignal West Dura Stable Peroxide Solution, 5 mL, store at room temperature
BACKGROUND
Description
O-GlcNAc Western Blot Detection Kit contains a monoclonal antibody which has specific affinity for the beta-O-linked serine or threonine GlcNAc modification. There is no cross-reactivity with the alpha-O-GlcNAc linkage, the α/β-O-GalNAc modification or other N-linked oligosaccharides. The kit also includes cell lysis reagent, secondary antibody and chemiluminescent substrate needed to perform a complete detection experiment.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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