CBGlyco™ Plus Activated Peroxidase HRP Labeling Kit (CAT#: Glyco-033CL)

Plus Activated Peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins. Each kit contains 5 x 1 mg of lyophilized HRP and sufficient Sodium cyanoborohydride solution, quenching buffer, and buffers to conjugate 5 mg of Immunoglobulin (IgG)


Specific Inquiry
  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 5 reactions
  • Label or Dye
  • HRP (Horseradish Peroxidase)
  • Labeling Scale
  • 0.5-1 mg
  • Labeling Target
  • Proteins (General), Antibodies (General)
  • Contents & storage
  • Sufficient For: 5 enzyme-labeling reactions, each with up to 1 mg of antibody (IgG)
    • Plus Activated Peroxidase, 5 x 1 mg
    • Sodium cyanoborohydride solution, 1 x 0.5 mL
    • Quenching Buffer, 25 mL
    • PBS Pack (makes 500 mL), 1 pack
    • Carbonate-Bicarbonate Buffer Pack (makes 500 mL), 1 pack

    Store at -20°C.

Background

  • Description
  • Plus Activated Peroxidase is horseradish peroxidase (HRP) enzyme, whose native carbohydrates (sugars) have been gently oxidized with periodate to produce amine-reactive aldehyde groups. These carbonyls of Plus Activated Peroxidase spontaneously and efficiently crosslink with primary amines on antibody or other proteins. The method is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and a greater degree of conjugate inactivation. The pre-activated enzyme eliminates the difficulties inherent in preparing and validating activated peroxidase from scratch. The kit provides the enzyme, accessory reagents and protocol to easily produce high-performance conjugates for Western blotting, ELISA and other detection techniques.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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