Products

CBGlyco™ Plus Activated Peroxidase HRP Labeling Kit (Glyco-033CL)

Plus Activated Peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins. Each kit contains 5 x 1 mg of lyophilized HRP and sufficient Sodium cyanoborohydride solution, quenching buffer, and buffers to conjugate 5 mg of Immunoglobulin (IgG)      

Specifications
Product Size
5 reactions
Label or Dye
HRP (Horseradish Peroxidase)
Labeling Scale
0.5-1 mg
Labeling Target
Proteins (General), Antibodies (General)
Contents & storage
Sufficient For: 5 enzyme-labeling reactions, each with up to 1 mg of antibody (IgG)
• Plus Activated Peroxidase, 5 x 1 mg
• Sodium cyanoborohydride solution, 1 x 0.5 mL
• Quenching Buffer, 25 mL
• PBS Pack (makes 500 mL), 1 pack
• Carbonate-Bicarbonate Buffer Pack (makes 500 mL), 1 pack

Store at -20°C.
BACKGROUND
Description
Plus Activated Peroxidase is horseradish peroxidase (HRP) enzyme, whose native carbohydrates (sugars) have been gently oxidized with periodate to produce amine-reactive aldehyde groups. These carbonyls of Plus Activated Peroxidase spontaneously and efficiently crosslink with primary amines on antibody or other proteins. The method is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and a greater degree of conjugate inactivation. The pre-activated enzyme eliminates the difficulties inherent in preparing and validating activated peroxidase from scratch. The kit provides the enzyme, accessory reagents and protocol to easily produce high-performance conjugates for Western blotting, ELISA and other detection techniques.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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