Products

CBGlyco™ Protein Stain (sensitive, R-250 coomassie stain) (Glyco-041CL)

Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels.      

Specifications
Product Size
1 L
3 x 1 L
Target Molecule
Protein
Label or Dye
Coomassie
Detection Method
Colorimetric
Detection Location
In-Gel Detection, In-Blot Detection
Contents & storage
Upon receipt store product at room temperature.
BACKGROUND
Description
Protein Stain is a coomassie dye reagent for detection of protein bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 2D gels. The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The Protein Stain protocol uses simple water-wash steps rather than methanol/acetic acid fixation and destaining, which saves valuable preparation time and minimizes reagent costs.

Protein stain is a unique formulation of Coomassie Brilliant Blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercially available coomassie-based stains. The easy-to-follow protocol is flexible to meet demanding time and sensitivity requirements. The use of Protein Stain does not result in the problems (e.g., staining consistency) that can be associated with other coomassie G-250 stain preparations. In addition to faster protein band development and improved sensitivity over standard coomassie G-250 stains, Protein Stain does not require methanol/acetic acid fixation and destaining, saving valuable preparation time and minimizing reagent cost.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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