R-Phycoerythrin AffiniPure F(ab')₂ Fragment Goat Anti-Human IgG, F(ab')₂ fragment specific (CAT#: MOB-0239MC)

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against the Fc portion of human IgG or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.


Specific Inquiry
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  • Datasheet
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Specifications

  • Host Species
  • Goat
  • Species Reactivity
  • Human
  • Conjugate
  • R-Phycoerythrin

Product Property

  • Purification
  • The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
  • Format
  • Freeze-dried powder
  • Buffer
  • 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
  • Storage
  • Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Store at 2-8°C - DO NOT FREEZE. Prepare working dilution fresh each day.

Applications

  • Application Notes
  • Flow Cytometry:- 1:50-1:200

Target

  • Alternative Names
  • F(ab')₂; fragment antigen-binding (Fab) fragment

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Mouse Antibody

CAT Product Name Application Type
MOB-0361MC Goat Anti-Human IgG Fab Antibody (HRP) ELISA, WB, IHC, IF/ICC

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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