Ranibizumab (CAT#: TP-104CL)
Ranibizumab is a recombinant humanized IgG1 kappa isotype monoclonal antibody fragment designed for intraocular use. Ranibizumab binds to and inhibits the biologic activity of human vascular endothelial growth factor A (VEGF-A). Ranibizumab has a molecular weight of approximately 48 kilodaltons and is produced by an E. coli expression system in a nutrient medium containing the antibiotic tetracycline (tetracycline is not detectable in the final product). Ranibizumab is marketed under the name ranibizumab. It is indicated for the treatment of macular edema after retinal vein occlusion, age-related macular degeneration (wet), and diabetic macular edema.
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WB
Figure 1 Anti-Human VEGF Antibody Fab Fragment (TP-104CL) in WB
Western blot analysis of TP-104CL was performed with Recombinant Human VEGF protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TP-104CL and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
ELISA
Figure 2 Anti-Human VEGF Antibody Fab Fragment (TP-104CL) in ELISA
ELISA analysis of TP-104CL was performed by coating with Recombinant Human VEGF Protein. Then blocked with BSA, and incubated with Anti-VEGF Therapeutic Antibody (ranibizumab) (TP-104CL). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
DB
Figure 3 Anti-Human VEGF Antibody Fab Fragment (TP-104CL) in DB
Antigen: Human VEGF protein
Antibody incubation concentration: 2ng/μL.
Specifications
- Type
- Biotech
- Trade name
- ranibizumab
- UNII
- ZL1R02VT79
Target
- Function
- Humanized mAb fragment that binds isoforms of vascular endothelial growth factor A (VEGFA)
Related Resources
Please refer to Ranibizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Ranibizumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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