Recombinant Human Anti-C5 Antibody scFv Fragment (CAT#: DrMAB-934)
Recombinant Human Antibody binds selectively to Human C5.
Specific Inquiry
ELISA
Figure 1 Phage display selected human scFvs against complement C5.
As shown by ELISA, 239 randomly selected clones from the fifth round of selection reacted with complement C5.
Zhu, W., Wang, Z., Hu, S., Gong, Y., Liu, Y., Song, H., ... & Yan, Y. (2019). Human C5-specific single-chain variable fragment ameliorates brain injury in a model of NMOSD. Neurology-Neuroimmunology Neuroinflammation, 6(3), e561.
ELISA
Figure 2 Human scFvs binds to complement C5.
C5B3 binds specifically to complement C5, but had a low affinity for control proteins (i.e., BSA and complements C3 and C1Q).
Zhu, W., Wang, Z., Hu, S., Gong, Y., Liu, Y., Song, H., ... & Yan, Y. (2019). Human C5-specific single-chain variable fragment ameliorates brain injury in a model of NMOSD. Neurology-Neuroimmunology Neuroinflammation, 6(3), e561.
SPR
Figure 3 Phage display selected human scFvs against complement C5.
The SPR sensorgram shows concentration-dependent binding of C5B3 to C5.
Zhu, W., Wang, Z., Hu, S., Gong, Y., Liu, Y., Song, H., ... & Yan, Y. (2019). Human C5-specific single-chain variable fragment ameliorates brain injury in a model of NMOSD. Neurology-Neuroimmunology Neuroinflammation, 6(3), e561.
Inhib
Figure 4 C5B3 prevented AQP4-IgG–mediated CDC without affecting AQP4-IgG binding to AQP4.
CDC in M23-AQP4–overexpressing CHO cells incubated with 2.5% hC and different concentrations of serum NMOSD and C5B3.
Zhu, W., Wang, Z., Hu, S., Gong, Y., Liu, Y., Song, H., ... & Yan, Y. (2019). Human C5-specific single-chain variable fragment ameliorates brain injury in a model of NMOSD. Neurology-Neuroimmunology Neuroinflammation, 6(3), e561.
Block
Figure 5 C5B3 blocked MAC formation.
Incubated M23-AQP4–overexpressing CHO cells with 10% serum NMOSD and 2.5% hC with or without 500 μg/mL of C5B3 for 30 minutes and fixed and stained them with anti–C5b-9 antibody.The red-to-blue fluorescence ratio to be statistically significant.
Zhu, W., Wang, Z., Hu, S., Gong, Y., Liu, Y., Song, H., ... & Yan, Y. (2019). Human C5-specific single-chain variable fragment ameliorates brain injury in a model of NMOSD. Neurology-Neuroimmunology Neuroinflammation, 6(3), e561.
Block
Figure 6 C5B3 decreased NMOSD mouse model lesions in vivo.
Lesion size quantification from experiments.
Zhu, W., Wang, Z., Hu, S., Gong, Y., Liu, Y., Song, H., ... & Yan, Y. (2019). Human C5-specific single-chain variable fragment ameliorates brain injury in a model of NMOSD. Neurology-Neuroimmunology Neuroinflammation, 6(3), e561.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Type
- scFv
- Specificity
- C5
- Species Reactivity
- Multiple
- Applications
- ELISA, SPR, Inhib
Product Property
- Purity
- >95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.
Applications
- Application Notes
- The C5 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Surface Plasmon Resonance, Inhibition.
Target
- Alternative Names
- C5; complement component 5; complement C5; CPAMD4; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; FLJ17816; FLJ17822; MGC142298
- Gene ID
- 727
Related Resources
Systemic Lupus Erythematosus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "C5"
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1372CL | Human Anti-C5 Recombinant Antibody (TAB-1372CL) | Inhib, FuncS | Humanized antibody |
| TAB-1373CL | Human Anti-C5 Recombinant Antibody (TAB-1373CL) | IB, CDC, Inhib | Humanized IgG |
| TAB-1374CL | Human Anti-C5 Recombinant Antibody (TAB-1374CL) | ELISA, CDC, Inhib | Humanized IgG |
| TAB-1375CL | Human Anti-C5 Recombinant Antibody (TAB-1375CL) | CDC, Inhib | Humanized IgG |
| TAB-1376CL | Human Anti-C5 Recombinant Antibody (TAB-1376CL) | CDC, Inhib | Humanized IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1370CL-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (TAB-1370CL-S(P)) | ELISA | Mouse scFv |
| TAB-1371CL-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (TAB-1371CL-S(P)) | Inhib, FuncS | Mouse scFv |
| TAB-1382CL-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (TAB-1382CL-S(P)) | ELISA, Inhib | Mouse scFv |
| TAB-1383CL-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (TAB-1383CL-S(P)) | ELISA, Inhib | Mouse scFv |
| TAB-1383CL-F(E) | Mouse Anti-C5 Recombinant Antibody; Fab Fragment (TAB-1383CL-F(E)) | ELISA, Inhib | Mouse Fab |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-082CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL249) | ELISA, Neut | Mouse IgG2a |
| NEUT-083CQ | Mouse Anti-C5 Recombinant Antibody (clone MM0608-6H13) | ELISA, Neut, WB | Mouse IgG1 |
| NEUT-084CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL030) | ELISA, WB, Neut | Mouse IgG1 |
| NEUT-085CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL250) | ELISA, WB, IHC, FuncS | Mouse IgG1 |
| NEUT-086CQ | Mouse Anti-C5 Recombinant Antibody (clone G25/2) | ELISA, IP, WB, Neut | Mouse IgG1, κ |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-088CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL771) | WB, Block, ELISA | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4104 | Hi-Affi™ Rabbit Anti-C5 Recombinant Antibody (clone SI175DS) | IHC-P | Rabbit IgG |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-453CQ | Anti-Human C5 Recombinant Antibody (Tesidolumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, λ2 |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0609-CN | Human Anti-C5 Recombinant Antibody (HPAB-0609-CN) | ELISA, Block | Humanized IgG |
| HPAB-602-FY | Human Anti-C5 Recombinant Antibody (HPAB-602-FY) | ELISA, Cyt, Inhib | Human IgG |
| HPAB-1484-FY | Mouse Anti-C5 Recombinant Antibody (HPAB-1484-FY) | ELISA, Neut | Mouse IgG |
| HPAB-1485-FY | Mouse Anti-C5 Recombinant Antibody (HPAB-1485-FY) | ELISA, Neut | Mouse IgG |
| HPAB-1577WJ | Mouse Anti-C5 Recombinant Antibody (clone 5G1.1) | ELISA, WB | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0613-CN-F(E) | Human Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0613-CN-F(E)) | WB, ELISA, FC | Humanized Fab |
| HPAB-0614-CN-F(E) | Mouse Anti-C5 Recombinant Antibody (clone m5G1.1); Fab Fragment | WB, ELISA, IF, IP, Block | Mouse Fab |
| HPAB-0615-CN-F(E) | Human Anti-C5 Recombinant Antibody (clone 5G1.1CB); Fab Fragment | WB, ELISA, IF, IP | Humanized Fab |
| HPAB-0123-LS-F(E) | Mouse Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0123-LS-F(E)) | Block, ELISA | Mouse Fab |
| HPAB-0124-LS-F(E) | Mouse Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0124-LS-F(E)) | Block, ELISA | Mouse Fab |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-559 | Recombinant Llama Anti-C5 Single Domain Antibody (Nb36) | ELISA | Llama VHH |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-462 | Human Anti-C5 Recombinant Antibody (clone SKY59/RO7112689); scFv Fragment | ELISA, WB, FC, Neut | Human scFv |
| HPAB-1484-FY-F(E) | Mouse Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-1484-FY-F(E)) | ELISA, Neut | Mouse Fab |
| HPAB-1485-FY-F(E) | Mouse Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-1485-FY-F(E)) | ELISA, Neut | Mouse Fab |
| HPAB-0527-YJ-S(P) | Human Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-0527-YJ-S(P)) | ELISA, Inhib, FuncS | Humanized scFv |
| HPAB-1577WJ-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (clone 5G1.1) | ELISA, WB | Mouse scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-453CQ | Afuco™ Anti-C5 ADCC Recombinant Antibody (Tesidolumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-008 | Afuco™ Anti-C5 ADCC Recombinant Antibody (Eculizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| AFC-TAB-436CQ | Afuco™ Anti-C5 ADCC Recombinant Antibody (Olendalizumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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