Recombinant Human Anti-HCV Core Protein Antibody scFv Fragment (CAT#: DrMAB-943)

Figure 1 Binding of individual phage clones harboring scFv fragments to HCV core protein.

Their reactivity with core protein (aa 1-115) relative to BSA was determined by phage EIA. Clones scFv41C, scFv42C, scFv50C, and scFv29C were thus identified as core protein binders.

Karthe, J., Tessmann, K., Li, J., Machida, R., Daleman, M., Häussinger, D., & Heintges, T. (2008). Specific targeting of hepatitis C virus core protein by an intracellular single‐chain antibody of human origin. Hepatology, 48(3), 702-712.

Figure 2 Purification of functional scFv42C.

Detection of purified scFv42C. Coomassie blue staining of purified scFv42C separated in SDS-PAGE.

Karthe, J., Tessmann, K., Li, J., Machida, R., Daleman, M., Häussinger, D., & Heintges, T. (2008). Specific targeting of hepatitis C virus core protein by an intracellular single‐chain antibody of human origin. Hepatology, 48(3), 702-712.

Figure 3 scFv42C binds to HCV core protein.

Binding of bacterially expressed and purified scFv42C fragment to HCV core protein.

Karthe, J., Tessmann, K., Li, J., Machida, R., Daleman, M., Häussinger, D., & Heintges, T. (2008). Specific targeting of hepatitis C virus core protein by an intracellular single‐chain antibody of human origin. Hepatology, 48(3), 702-712.

Figure 4 Colocalization of HCV core protein with scFv42C.

Colocalization was confirmed by merging core and scFv42C images

Karthe, J., Tessmann, K., Li, J., Machida, R., Daleman, M., Häussinger, D., & Heintges, T. (2008). Specific targeting of hepatitis C virus core protein by an intracellular single‐chain antibody of human origin. Hepatology, 48(3), 702-712.

Figure 5 FRET analysis.

Specific interaction between scFv42C and HCV core protein.

Karthe, J., Tessmann, K., Li, J., Machida, R., Daleman, M., Häussinger, D., & Heintges, T. (2008). Specific targeting of hepatitis C virus core protein by an intracellular single‐chain antibody of human origin. Hepatology, 48(3), 702-712.

Figure 6 Effect of scFv42C on core protein expression, replication, and cell proliferation.

Immune staining of core protein expressed from JFH1 replicon. Cells were fixed 3 days later and stained with anti-core antibody followed by ultraviolet microscopy.

Karthe, J., Tessmann, K., Li, J., Machida, R., Daleman, M., Häussinger, D., & Heintges, T. (2008). Specific targeting of hepatitis C virus core protein by an intracellular single‐chain antibody of human origin. Hepatology, 48(3), 702-712.

Figure 7 scFv42C inhibits cell proliferation.

Effect of scFv42C on cell proliferation. Cell proliferation was measured by a modified MTT assay (cholecystokinin-8 assay) at day 1 after transfection.

Karthe, J., Tessmann, K., Li, J., Machida, R., Daleman, M., Häussinger, D., & Heintges, T. (2008). Specific targeting of hepatitis C virus core protein by an intracellular single‐chain antibody of human origin. Hepatology, 48(3), 702-712.