Recombinant Mouse Anti-Human CD32 Antibody F(ab')2 Fragment, clone 7.3 (CAT#: DrMAB-982)
Recombinant Mouse Antibody binds selectively to Mouse FCGR2A, expressed in Raji.
Specific Inquiry
Block
Figure 1 Effect of FcgR blocking on ADCP.
Flow cytometry was used to calculate the percentage of tumour cell engulfment by cultured macrophages in the presence of 10 μg/ml of uhPR1A3. Fab2 fragments were used to block either FcgR I (CD64), FcgR II (CD32) or FcgR III (CD16) (each antibody concentration was1 mg ml1).
Ashraf, S., Q., Umana, P., & Mossner, et al. (2009). Humanised igg1 antibody variants targeting membrane-bound carcinoembryonic antigen by antibody-dependent cellular cytotoxicity and phagocytosis. BRITISH JOURNAL OF CANCER.
FC
Figure 2 Expression of activating and inhibitory Fc Rs on human monocyte-derived DCs.
Surface expression of Fc RI (22.2-FITC) and Fc RII (IV.3-FITC and 7.3-FITC) on Ma, iDC, and mDC as assessed by flow cytometry.
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
FC
Figure 3 Phagocytosis and TNF-α production mediated by activating and inhibitory Fc R in human DCs.
Phagocytosis of PKH26-labeled probes E-22.2, E-IV.3, and E-7.3 was measured by flow cytometry.
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
ELISA
Figure 4 Phagocytosis and TNF-α production mediated by activating and inhibitory Fc R in human DCs.
Secretion of TNF-α by iDC cultured for 18 h E-IV.3 probes and E-7.3 probes as compared with E without Ab was assessed by ELISA.
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
IF
Figure 5 Internalization of MP-coated Fc R-specific phagocytic probes by DCs.
Internalized MP following uptake of E-MP-7.3 by mDC was detected in immunofluorescence microscopy with rabbit anti-MP Abs and Alexa 546-conjugated goat antirabbit IgG counterstained with 7.3-FITC.
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
ELISPOT
Figure 6 Differential expression of Fc R on DC alters cross-presentation of influenza MP to memory T cells.
ELISPOT assay for IFN-α-producing influenza memory T cells cultured with iDCs following uptake of 9-mer and 17-mer coupled to IV.3 probes (E-MP-IV.3) and 7.3 probes (E-MP-7.3).
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
Inhib
Figure 7 Cytokine regulation of Fc RII-mediated phagocytosis in mDCs.
Percent inhibition in the PI of E-7.3 probes compared with E-IV.3 probes measured in mDCs cultured in complete medium, IL-10, IL-13, and IL-13 plus IL-10.
Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., & Pricop, L. (2006). Regulated expression of FcγR in human dendritic cells controls cross-presentation of antigen-antibody complexes. The Journal of Immunology, 177(12), 8440-8447.
Specifications
- Immunogen
- Human K562 tumor cells and L cells transfected with Human Fc gamma RII
- Host Species
- Mouse
- Type
- F(ab')2
- Specificity
- Antibody reacts with a domain 2 epitope of all CD32 isoforms. It exhibits preferential binding to Fc gRIIb (inhibitory) isoform transfectants.
- Species Reactivity
- Human
- Clone
- 7.3
- Applications
- Block, FC, ELISA, IF, ELISPOT
Product Property
- Purification
- Protein A purification
- Purity
- >95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.
- Concentration
- 1 mg/ml
- Buffer
- 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added
- Storage
- Store at 2-5°C. Open under aseptic conditions.
Applications
- Application Notes
- The FCGR2A antibody has been reported in applications of Blocking, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Enzyme-linked Immunosorbent Spot.
Target
- Alternative Names
- FCGR2A; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32), FCG2, FCGR2, FCGR2A1; low affinity immunoglobulin gamma Fc region receptor II-a; CD32; CD32A; CDw32; IGFR2; Immunoglobulin G Fc recep
- Gene ID
- 2212
- UniProt ID
- P12318
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-983 | Mouse Anti-FCGR2A Recombinant Antibody (clone 7.3); Fab Fragment | FC | Mouse Fab |
| TAB-039WM-F(E) | Human Anti-FCGR2A Recombinant Antibody (clone MDE-8); Fab Fragment | FC, WB, ELISA, Inhib | Human Fab |
| HPAB-0223-YC-F(E) | Mouse Anti-FCGR2A Recombinant Antibody (clone mAb IV.3); Fab Fragment | ELISA, FuncS | Mouse Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-039WM | Human Anti-FCGR2A Recombinant Antibody (clone MDE-8) | FC, WB, ELISA, Inhib | Human IgG |
| MRO-0295-CN | Rabbit Anti-FCGR2A Recombinant Antibody (clone JM10-70) | WB, IHC | Rabbit IgG |
| FN-009CQ | Mouse Anti-FCGR2A Recombinant Antibody (clone 7.3) | FC, Block | Mouse IgG1, κ |
| FN-172CQ | Mouse Anti-FCGR2A Recombinant Antibody (clone 6C4) | FC, FuncS, Neut | Mouse IgG1, κ |
| VS3-FY540 | Recombinant Rabbit Anti-FCGR2A Antibody (clone R09-5D1) | WB, IHC-P | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-039WM-S(P) | Human Anti-FCGR2A Recombinant Antibody (clone MDE-8); scFv Fragment | FC, WB, ELISA, Inhib | Human scFv |
| HPAB-0223-YC-S(P) | Mouse Anti-FCGR2A Recombinant Antibody (clone mAb IV.3); scFv Fragment | ELISA, FuncS | Mouse scFv |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-790CQ | Mouse Anti-FCGR2A Recombinant Antibody (clone IV.3) | Block | Mouse IgG2b |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-791CQ | Mouse Anti-FCGR2A Recombinant Antibody (clone AT10) | Neut, FC, IHC-Fr, IP | Mouse IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1272 | Hi-Affi™ Rabbit Anti-FCGR2A Recombinant Antibody (clone DS1272AB) | WB, IHC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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