Recombinant Mouse Anti-Indolicidin Antibody Fab Fragment (V2D2) (CAT#: PABX-117-F (E))

Anti-Indolicidin antibody is a Mouse of Fab class that binds to an Indolicidin. This antibody bounds indolicidin with nanomolar affinity (KD=56 nM) and could block the antibacterial action of the peptide.


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Figure 1 ELISA profile of a panel of anti-indolicidin hybridomas. Clones marked in black were selected for further investigation.

Figure 1 ELISA profile of a panel of anti-indolicidin hybridomas. Clones marked in black were selected for further investigation.

Lomash, S., Nagpal, S., & Salunke, D. M. (2010). An antibody as surrogate receptor reveals determinants of activity of an innate immune peptide antibiotic. Journal of Biological Chemistry, 285(46), 35750-35758.

Figure 2 Surface plasmon resonance (SPR) sensorgram for the binding of mAb V2D2 (analyte; concentration ranging from 20 to 320 nM) to indolicidin (ligand) immobilized on a CM4 chip.

Figure 2 Surface plasmon resonance (SPR) sensorgram for the binding of mAb V2D2 (analyte; concentration ranging from 20 to 320 nM) to indolicidin (ligand) immobilized on a CM4 chip.

Association (ka1), dissociation (kd1) rate constants were used to calculate equilibrium constant (KD).

Lomash, S., Nagpal, S., & Salunke, D. M. (2010). An antibody as surrogate receptor reveals determinants of activity of an innate immune peptide antibiotic. Journal of Biological Chemistry, 285(46), 35750-35758.

Figure 3 Structural features of Fab V2D2.

Figure 3 Structural features of Fab V2D2.

A, a schematic rendering of crystal structure of Fab V2D2. CDRs are colored red. B, surface model looking through the long axis at CDR orientation, colored according to electrostatic potential. Probable antigen binding site ismarked with a cyan arrow.C, orientation of CDRH3 upon peptide binding in the closely homologous structure PDB 2BRR (molecule LH, gray) was used to model an alternate conformation of the same loop in Fab V2D2, colored according to B-factors (red to blue indicate high to low).

Lomash, S., Nagpal, S., & Salunke, D. M. (2010). An antibody as surrogate receptor reveals determinants of activity of an innate immune peptide antibiotic. Journal of Biological Chemistry, 285(46), 35750-35758.


Specifications

  • Immunogen
  • Indolicidin conjugated to diphtheria toxoid
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Fab
  • Specificity
  • Tested positive against native Indolicidin
  • Clone
  • V2D2
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:10000-1:20000
    Neut (assay dependent)
    FuncS (assay dependent)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

CAT Product Name Application Type
MOB-573 Recombinant Anti-Indolicidin Antibody ELISA, IP, FuncS IgG
MHH-573 Recombinant Human Anti-Indolicidin Antibody WB, FC, FuncS IgG

Fab Fragment Antibody

scFv Fragment Antibody

CAT Product Name Application Type
MOB-573-S(P) Recombinant Anti-Indolicidin Antibody scFv Fragment ELISA, WB, FC, FuncS scFv
MHH-573-S(P) Recombinant Human Anti-Indolicidin Antibody scFv Fragment ELISA, WB, IP, FuncS scFv

Mouse Antibody

CAT Product Name Application Type
PABX-117 Recombinant Mouse Anti-Indolicidin Antibody (V2D2) WB, ELISA, Neut, FuncS IgG
PABX-117-S (P) Recombinant Mouse Anti-Indolicidin Antibody scFv Fragment (V2D2) WB, ELISA, Neut, FuncS scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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