Recombinant Mouse Anti-Integrin alpha 4 Antibody Fab Fragment (ACT-1) (CAT#: PABX-122-F (E))

Figure 1 Nuclear localization of Act1

HBE1 cells plated on glass coverslips were left unstimulated or stimulated with 100 ng/mL IL-17A for 1h. Endogenous Act1 was immunostained with a rabbit anti-Act1 antibody, followed by an Alexa-488 conjugated secondary antibody (green) and counterstained with DAPI to visualize nuclei (blue).

Velichko, S., Zhou, X., Zhu, L., Anderson, J. D., Wu, R., & Chen, Y. (2016). A novel nuclear function for the interleukin-17 signaling adaptor protein Act1. PloS one, 11(10), e0163323.

Figure 2 Binding of mAb ACT-1, HIV-1 gp120, and cyclic V2 peptides to RMPI8866 cells.

(A) RPMI8866 cells (0.5 x106) were incubated with 1 μg APC labeled ACT-1 (α4β7 specific antibody). Antibody binding was measured by flow cytometry and is presented as a histogram. Over 99% of the RPMI8866 cells expressed α4β7 integrin. Unstained cells (grey shading) and isotype control antibody (solid line) were used as controls. (A and C) Histograms show the binding of CHO-expressed HIV-1 gp120 (2.5 μg, blue line panel B), biotin labeled cyclic V2 TH023 peptide (1μg and 5 μg; black and orange lines, respectively; panel C), and biotin labeled cyclic V2 TH023 -fully scrambled peptide (SCR; 1 μg and 5 μg; blue line and green line; panel C) to RMPI8866 cells. Polyclonal mouse anti-gp145 antibody followed by goat anti-mouse-Texas Red was used to visualize the gp120 protein binding while the V2 peptides were visualized with neutrAvidin-PE. Cells treated with only anti-mouse-Texas Red (red line, panel B) and unstained cells (red line, panel C) were used as controls in addition to the fully scrambled V2 peptide (blue and green line, panel C). Representative histograms are shown for panels A, B, and C (n = 2).

Peachman, K. K., Karasavvas, N., Chenine, A. L., McLinden, R., Rerks-Ngarm, S., Jaranit, K., ... & Michael, N. L. (2015). Identification of new regions in HIV-1 gp120 variable 2 and 3 loops that bind to α4β7 integrin receptor. PLoS One, 10(12), e0143895.

Figure 3 Immunohistochemical staining of RAGE, Act1 and IL-17.

(a) The synovial tissue sections from patients with rheumatoid arthritis and osteoarthritis were stained with antibodies to RAGE, Act-1, IL-17, and H&E or an isotype-control antibody. The brown color shows the target.

Heo, Y. J., Oh, H. J., Jung, Y. O., Cho, M. L., Lee, S. Y., Yu, J. G., ... & Kim, H. Y. (2011). The expression of the receptor for advanced glycation end-products (RAGE) in RA-FLS is induced by IL-17 via Act-1. Arthritis research & therapy, 13(4), R113.