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TEM-1 β-lactamase scaffold protein anti-Human β-Gal (CAT#: STE-L197)

The TEM-1 β-lactamase that bound β-Gal was isolated from the library using Phage display techonology. After affinity maturation, TEM-1 β-lactamase variants were identified having affinities for β-Gal as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/814_D2_1_selected.jpg
Figure 2 IHC staining of human testis
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in leydig cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8382/ihc_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/759_D2_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/755_D2_1_blue_red_green.jpg
Normal Tissue
Figure 5 Thyroid gland
Figure 5 Thyroid gland
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_B_1_5.jpg
Figure 6 Colon
Figure 6 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_7_3.jpg
Figure 7 Liver
Figure 7 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BEWO)
Figure 9 RNA cell line category: Cell line enhanced (BEWO)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170266-GLB1

Specifications

  • Scaffold Name
  • TEM-1 β-lactamase
  • Origin
  • TEM-1 β-lactamase
  • Core Structure
  • α-Helixes, β-sheets
  • Variable Regions
  • 1–2 Loops
  • Target
  • β-Gal
  • Species Reactivity
  • Human
  • Expression Host
  • BL21 (DE3) cells
  • Applications
  • ELISA; IHC; Microscopy; FC; WB; FuncS

Target

  • Alternative Names
  • TEM β-lactamase; TEM-1 β-lactamase; EBP; ELNR1; MPS4B

Downloads

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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