Anti-Human Integrin alpha 5 beta 1 Recombinant Antibody (Volociximab) (CAT#: TAB-077)

Recombinant monoclonal antibody to Integrin Integrin alpha 5 beta 1. Volociximab (also known as M200) is a chimeric monoclonal antibody jointly for treatment of a variety of advanced solid tumors. It binds to and inhibits the functional activity of α5β1 integrin.
It is thought to reduce metastases. Early results show potential in renal cell cancers.


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  • COA
IHC

Figure 1 Testing antibody cross-reactivity to human α5β1.

Figure 1 Testing antibody cross-reactivity to human α5β1.

IIA1, the mouse parent antibody of volociximab, which recognizes only human integrin, anti-mouse CD31, which stains mouse vessels, and pooled rat IgG are shown as controls.

Bhaskar, V., Zhang, D., Fox, M., Seto, P., Wong, M. H., Wales, P. E., ... & Ramakrishnan, V. (2007). A function blocking anti-mouse integrin α5β1 antibody inhibits angiogenesis and impedes tumor growth in vivo. Journal of translational medicine, 5(1), 61.

ELISA

Figure 2 Representative antibodies were compared by ELISA for binding to human and murine α5β1-Fc or irrelevant Fc fusion proteins.

Figure 2 Representative antibodies were compared by ELISA for binding to human and murine α5β1-Fc or irrelevant Fc fusion proteins.

A subset of competitive antibodies cross-reacted with human integrin.

Bhaskar, V., Zhang, D., Fox, M., Seto, P., Wong, M. H., Wales, P. E., ... & Ramakrishnan, V. (2007). A function blocking anti-mouse integrin α5β1 antibody inhibits angiogenesis and impedes tumor growth in vivo. Journal of translational medicine, 5(1), 61.

ELISA

Figure 3 Binding of volociximab to integrin α5β1 and inhibition of integrin binding to fibronectin.

Figure 3 Binding of volociximab to integrin α5β1 and inhibition of integrin binding to fibronectin.

A dilution series of volociximab was incubated with immobilized human, mouse or rabbit integrin α5β1- Fc fusion proteins and bound volociximab was detected using an anti-human κ-chain specific antibody.

Bhaskar, V., Fox, M., Breinberg, D., Wong, M. H., Wales, P. E., Rhodes, S., ... & Ramakrishnan, V. (2008). Volociximab, a chimeric integrin alpha5beta1 antibody, inhibits the growth of VX2 tumors in rabbits. Investigational new drugs, 26(1), 7-12.

IHC

Figure 4 VX2 prevention study. Thirty rabbits were inoculated with subcutaneous tumors on the left hind limb.

Figure 4 VX2 prevention study. Thirty rabbits were inoculated with subcutaneous tumors on the left hind limb.

Volociximab-treated rabbits generally grew fewer tumor-associated vessels than vehicle-treated rabbits

Bhaskar, V., Fox, M., Breinberg, D., Wong, M. H., Wales, P. E., Rhodes, S., ... & Ramakrishnan, V. (2008). Volociximab, a chimeric integrin alpha5beta1 antibody, inhibits the growth of VX2 tumors in rabbits. Investigational new drugs, 26(1), 7-12.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Chimeric (mouse/human)
  • Type
  • IgG4
  • Specificity
  • Tested positive against native human antigen.
  • Species Reactivity
  • Human
  • Applications
  • ELISA, IHC
  • CAS
  • 558480-40-3
  • Generic Name
  • Volociximab
  • UNII
  • 496K5Z02NW
  • MW
  • 145.5 kDa
  • Related Disease
  • Unknown

Product Property

  • Purity
  • >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The Integrin alpha 5 beta 1 antibody has been reported in applications of Immunohistochemistry, Enzyme-linked Immunosorbent Assay.

Target

  • Alternative Names
  • Volociximab;558480-40-3;M200;ITGA5;integrin, alpha 5 (fibronectin receptor, alpha polypeptide);FNRA;integrin alpha-5;CD49e;VLA-5;integrin alpha-F;CD49 antigen-like family member E;fibronectin receptor subunit alpha;fibronectin receptor, alpha subunit;very

Related Resources

  • Biosimilar Overview
Please refer to Volociximab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Volociximab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Volociximab"

Afuco™ Anti-ITGA5 ADCC Recombinant Antibody (Volociximab), ADCC Enhanced
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Integrin Integrin alpha 5 beta 1. Volociximab (also known as M200) is a chimeric monoclonal antibody jointly for treatment of a variety of advanced solid tumors. It binds to and inhibits the functional activity of α5β1 integrin.
It is thought to reduce metastases. Early results show potential in renal cell cancers.

See other products for "ITGA5"

Recombinant Antibody

Blocking Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1874 Hi-Affi™ Recombinant Rabbit Anti-ITGA5 Monoclonal Antibody (DS1874AB) WB, IHC-P, ICC/IF, IP, FC IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-077 Afuco™ Anti-ITGA5 ADCC Recombinant Antibody (Volociximab), ADCC Enhanced IP, IF, FuncS, FC, Neut, ELISA ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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