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As a leading technique provider in antibody engineering field, Creative Biolabs provides best in class CrossMabs services. Our scientists have broad experience in antibodies engineering and can offer you a sophisticated strategy tailored to your exact needs.

CrossMab (where Mab representative of Monoclonal Antibody) is a novel format of BsAbs derived from independent parental antibodies. This new technique can be used to produce a bispecific antibody (BsAb) with antibody domain exchange based on knobs-into-holes (KIH) method. This “crossover” keeps the antigen-binding affinity and also preserves the two different arms in order to avoid light-chain mispairing. In comparison with natural antibodies, CrossMAbs show higher stability. We have several different CrossMAb designs as in following diagram, such as Fab, VH-VL and CH1-CL exchanged in different regions. CrossMAbFab format exchanges the full VH-CH1 and VL-CL regions; CrossMAbVH-VL format exchanges the VH and VL regions only; CrossMAbCH1-CL format exchanges the CH1 and CL regions of bispecific antibody.

Figure:Schematic diagram of CrossMab generation (PNAS, 2011)

Our CrossMAb platform allows the development of bispecific antibodies with excellent stability and high affinity for two different targets. Experts from Creative Biolabs can design a suitable strategy which can greatly accelerate your research.

Schaefer, et al. Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies.Proc Natl Acad Sci U S A. 2011; 108(27):11187-92.

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