10-FnIII scaffold protein anti-SH3 domain of human c-Src
CAT#: SFN-L022
The tenth fibronectin type III domain (10Fn3) that bound SH3 domain of human c-Src was isolated from the library using Phage display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=0.25 μM) for SH3 domain of human c-Src as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/850_G12_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows membranous and cytoplasmic positivity in cells in seminferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_B_9_5.jpg
Normal Tissue
Figure 4 Stomach
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_1.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A549, HBEC3-KT, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197122-SRC
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Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2–3 loops
- Target
- SH3 domain of human c-Src
- Species Reactivity
- Human
- Expression Host
- E. coli
- Affinity Constant
- 0.25 μM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
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Related Signaling Pathways
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COA
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