10-FnIII scaffold protein anti-SH3 domain of human c-Src (CAT#: SFN-L022)
The tenth fibronectin type III domain (10Fn3) that bound SH3 domain of human c-Src was isolated from the library using Phage display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=0.25 μM) for SH3 domain of human c-Src as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/850_G12_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/850_G12_2_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows membranous and cytoplasmic positivity in cells in seminferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6_selected.jpg
(Immunohistochemical staining of human testis shows membranous and cytoplasmic positivity in cells in seminferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_B_9_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_B_9_5.jpg
Figure 4 Stomach
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_1.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_1.jpg
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_8_3.jpg
Figure 6 Kidney
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_7_5.jpg
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_7_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (A549, HBEC3-KT, RT4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197122-SRC
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197122-SRC
Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2–3 loops
- Target
- SH3 domain of human c-Src
- Species Reactivity
- Human
- Expression Host
- E. coli
- Affinity Constant
- 0.25 μM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
Related Resources
Bladder Cancer
Throid Cancer
Endocrine Resistance
cAMP Signaling Pathway
ErbB Signaling Pathway
MAPK Signaling Pathway
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3405 | Hi-Affi™ Recombinant Rabbit Anti-SRC Monoclonal Antibody (DS3405AB) | IP, WB, Dot, ICC/IF | IgG |
| MOR-4740 | Hi-Affi™ Recombinant Rabbit Anti-SRC Monoclonal Antibody (TH254DS) | WB, IF, ICC, FC | IgG |
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