APC-A*02:01/Human DDX5 (YLLPAIVHI) MHC Tetramer (CAT#: MHC-LC2359)
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of human DDX5 of peptide YLLPAIVHI covering 148-156 and A*02:01 molecule. The pMHC tetramer recognizes CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/278_G2_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/278_G2_1_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71154/1949_B4_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71154/1949_B4_1_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5868/642_D2_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5868/642_D2_1_selected.jpg
Figure 4 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_4_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_4_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_8_8.jpg
Figure 6 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45892_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45892_B_8_5.jpg
Figure 7 Colon
(Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_9_3.jpg
(Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_9_3.jpg
Figure 8 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_8_5.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108654-DDX5
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108654-DDX5
Specifications
- Allele
- A*02:01
- Class
- Class I
- MHC Species
- Human
- Antigen
- DDX5
- Antigen Species
- Human
- Peptide
- YLLPAIVHI
- Range
- 148-156
- Conjugate
- APC
- Application
- FCM
Target
- Antigen Introduction
- DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. Alternative splicing results in multiple transcript variants.
- Alternative Names
- DDX5; DEAD-box helicase 5; p68; HLR1; G17P1; HUMP68
- Gene ID
- 1655
- UniProt ID
- P17844
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2288CT | Recombinant Mouse anti-Human DDX5 Monoclonal antibody (70) | WB, Dot | |
| HPAB-0197-CN | Human Anti-DDX5 Recombinant Antibody (clone 1G3) | ELISA | Human IgG |
| MRO-0467-CN | Rabbit Anti-DDX5 Recombinant Antibody (clone JM52-30) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0634J | Rabbit Anti-DDX5 Recombinant Antibody (clone 2C3) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| VS3-FY449 | Recombinant Rabbit Anti-DDX5 Antibody (clone R04-2K2) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
Customer Reviews and Q&As
Customer Review
Q&As
Improves Multicolor Flow Cytometry
Its ability to be used with multiple fluorescent markers enhances our multicolor flow cytometry applications. This compatibility allows for comprehensive assessment of T cell characteristics, vital for our immune profiling efforts.
Reliable and Consistent Results
Providing reproducible performance, the tetramer is essential in longitudinal studies analyzing T cell functions over time, ensuring confidence in our findings related to immune-related disease models.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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