APC-A*24:02/Human CA9 (EYRALQLHL) MHC Tetramer

Name: APC-A*24:02/Human CA9 (EYRALQLHL) MHC Tetramer
SKU: MHC-LC176
Rating: 5 (1 reviews)

CAT#: MHC-LC176

This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of Carbonic Anhydrase 9-derived peptide of EYRALQLHL sequence covering 219-227 and A*24:02 molecule. The pMHC tetramer recognizes human CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.

Gene Expression

Normal Tissue Figure 1 Immunofluorescent staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5100/639_D5_2_selected.jpg

Normal Tissue Figure 2 Stomach

Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55207/139851_A_5_1.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (A-431, HDLM-2, hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000107159-CA9

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Specifications

Allele
A*24:02

Class
Class I

MHC Species
Human

Antigen
Carbonic Anhydrase 9

Antigen Species
Human

Peptide
EYRALQLHL

Range
219-227

Conjugate
APC

Application
FCM

Target

Antigen Introduction
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.

Alternative Names
CA9; carbonic anhydrase 9; MN; CAIX

Gene ID
911829

UniProt ID
Q05320

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Datasheet

Datasheet - MHC-LC176.pdf

MSDS

MSDS - MHC-LC176.pdf

COA

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